# Yokogawa CSU-X1 vs Crest X-Light V3 light output **URL:** https://forum.microlist.org/t/yokogawa-csu-x1-vs-crest-x-light-v3-light-output/1240 **Category:** Uncategorized **Created:** [December 18, 2020, 11:40pm UTC](https://forum.microlist.org/t/yokogawa-csu-x1-vs-crest-x-light-v3-light-output/1240 "2020-12-18T23:40:52Z") **Posts on this page:** 1 **Showing post:** 4
### Author: ![WillGiang](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/willgiang/32/1224_2.png) [@WillGiang](https://forum.microlist.org/u/WillGiang) #### Post date: [January 3, 2021, 4:08am UTC](https://forum.microlist.org/t/yokogawa-csu-x1-vs-crest-x-light-v3-light-output/1240/4 "2021-01-03T04:08:37Z")
I referenced your thread a lot as the [~$200k total](https://forum.microlist.org/t/crest-x-light-v3-spinning-disk-confocal-reviews/565/15) for everything you got was quite jaw-dropping! Do you mainly do fixed imaging where the atypical lines of the cheapest LDI doesn’t matter as much? Switching 470 to 488 and 555 to 577 would have added a fair amount to the price, and the main intended use case will be fast live cell imaging of weakly expressing FPs. The V3 does use microlenses–but as I understand it, they are solely for homogenizing the illumination. The X1’s microlenses boost excitation transmission AND reduce the amount of reflected light from the disk. I was worried that some of the stray light eventually makes it through the disk/objective to the sample, but CrestOptics says this isn’t the case. To CrestOptics’ credit, the high OD filters and variable irises (and supposedly the V3’s re-designed light path) do a good job of preventing this stray light from reaching the camera. The X1 has the benefit of the dichroic being placed between the dual disks. I didn’t fully appreciate the X1’s design until about a month ago. I just really wished that the X1 had a larger FOV. One additional consideration in our case are FRAP capabilities–as I understand it, those units need single-mode fibers, so buying extra lasers can get quite expensive, especially if 561 is among the list. Depending on who’s sourcing the lasers (and how many you need), utilizing a galvo switcher is possible and might close the cost difference gap between the X1 and V3. There’s a lot of extra info here, and it isn’t necessarily directed at anyone–I hope it’s helpful for future readers. --- _[View the full topic](https://forum.microlist.org/t/yokogawa-csu-x1-vs-crest-x-light-v3-light-output/1240)._