# Welcome to the Microtutor discussion forum

**URL:** <https://forum.microlist.org/t/welcome-to-the-microtutor-discussion-forum/2560>\
**Category:** Microtutor\
**Created:** [June 3, 2024, 7:37pm UTC](https://forum.microlist.org/t/welcome-to-the-microtutor-discussion-forum/2560 "2024-06-03T19:37:43Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![jennifer](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jennifer/32/634_2.png) [@jennifer](https://forum.microlist.org/u/jennifer)\
**Post date:** [June 3, 2024, 7:37pm UTC](https://forum.microlist.org/t/welcome-to-the-microtutor-discussion-forum/2560/1 "2024-06-03T19:37:43Z")

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Hi all! The Microtutor development team and instructors are monitoring this forum and are happy to answer your questions on the Fluorescence Microscopy course content! Who’s first? 🙂

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**Author:** ![kalaisekar](https://avatars.discourse-cdn.com/v4/letter/k/f0a364/32.png) [@kalaisekar](https://forum.microlist.org/u/kalaisekar)\
**Post date:** [September 5, 2024, 8:24am UTC](https://forum.microlist.org/t/welcome-to-the-microtutor-discussion-forum/2560/2 "2024-09-05T08:24:39Z")

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Microtutor is really a big boon for light microscopy enthusiast like me. Course contents look exiting. I think inclusion of a bit of technical aspects on plant and animal sample preparation for fluorescence microscopy could be of additional benefit to the learners.

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**Author:** ![yuexuan\_moriaty](https://avatars.discourse-cdn.com/v4/letter/y/7993a0/32.png) [@yuexuan\_moriaty](https://forum.microlist.org/u/yuexuan_moriaty)\
**Post date:** [October 12, 2024, 11:39am UTC](https://forum.microlist.org/t/welcome-to-the-microtutor-discussion-forum/2560/3 "2024-10-12T11:39:37Z")

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Thank you so much for the development team and instructors for making this amazing course! 🥰I have learnt a lot throughout the course!! I have one question regarding the content in chapter1. For the brightness and the spectra of a fluorophore, does it also depend on experimental factors (other than QE and EC), such as pH, temperature etc. And if so, where can I find relevant information, like can I find how the spectrum changes depending on pH in FPbase?