# W1 Primary Dichroic Misalignment

**URL:** <https://forum.microlist.org/t/w1-primary-dichroic-misalignment/1438>\
**Category:** Hardware\
**Created:** [June 14, 2021, 5:55pm UTC](https://forum.microlist.org/t/w1-primary-dichroic-misalignment/1438 "2021-06-14T17:55:18Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![EricGriffis](https://avatars.discourse-cdn.com/v4/letter/e/58f4c7/32.png) [@EricGriffis](https://forum.microlist.org/u/EricGriffis)\
**Post date:** [June 14, 2021, 5:55pm UTC](https://forum.microlist.org/t/w1-primary-dichroic-misalignment/1438/1 "2021-06-14T17:55:18Z")

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Does anyone have an easy way to align images taken with the two primary dichroic mirrors in the W1? I’ve found that there isn’t a great way to ensure that the hardware alignment between the 405-488-561-640 and 445-515-594 dichroics is spot on. So, we have to do post-imaging alignment when we are looking at fixed samples of DAPI-CFP-YFP-Cy5 or similar. Do you have a milled target that you image with both primaries in place and then come up with a shift correction? Do you use the parked disk? Do you have users include something like TetraSpeck beads for fiducial marks?