# Trichrome Stain

**URL:** <https://forum.microlist.org/t/trichrome-stain/2163>\
**Category:** Probes & Prep\
**Created:** [May 8, 2023, 5:07am UTC](https://forum.microlist.org/t/trichrome-stain/2163 "2023-05-08T05:07:05Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![BenAbrams](https://avatars.discourse-cdn.com/v4/letter/b/f0a364/32.png) [@BenAbrams](https://forum.microlist.org/u/BenAbrams)\
**Post date:** [May 8, 2023, 5:07am UTC](https://forum.microlist.org/t/trichrome-stain/2163/1 "2023-05-08T05:07:05Z")

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Hi,

Does anyone have any recommendations for getting good Trichrome staining. A user in my facility has asked about this and I have no experience with it. Prep tips or a suggestion for a good kit would be very helpful.

Thanks,  
Ben

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**Author:** ![smcardle](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/smcardle/32/155_2.png) [@smcardle](https://forum.microlist.org/u/smcardle)\
**Post date:** [May 9, 2023, 8:45pm UTC](https://forum.microlist.org/t/trichrome-stain/2163/2 "2023-05-09T20:45:48Z")

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Here’s a protocol we use for trichrome staining of FFPE sections: [Masson&#39;s Trichrome Staining Protocol for FFPE spheroids and microtissues](https://www.protocols.io/view/masson-39-s-trichrome-staining-protocol-for-ffpe-s-rm7vz82w5vx1/v1)

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**Author:** ![BenAbrams](https://avatars.discourse-cdn.com/v4/letter/b/f0a364/32.png) [@BenAbrams](https://forum.microlist.org/u/BenAbrams)\
**Post date:** [May 9, 2023, 10:27pm UTC](https://forum.microlist.org/t/trichrome-stain/2163/3 "2023-05-09T22:27:01Z")

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Thanks smcardle, I’ll have my user give that a shot, much appreciated.  
Ben
