# Transwell migration imaging

**URL:** <https://forum.microlist.org/t/transwell-migration-imaging/732>\
**Category:** Hardware\
**Created:** [November 25, 2019, 11:56am UTC](https://forum.microlist.org/t/transwell-migration-imaging/732 "2019-11-25T11:56:08Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![llemgruber](https://avatars.discourse-cdn.com/v4/letter/l/dfb087/32.png) [@llemgruber](https://forum.microlist.org/u/llemgruber)\
**Post date:** [November 25, 2019, 11:56am UTC](https://forum.microlist.org/t/transwell-migration-imaging/732/1 "2019-11-25T11:56:08Z")

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A user in our facility wants to do live imaging of cell migrating through a transwell. Does anyone has any tips on how to set-up the assay?  
Thanks! 🙂

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**Author:** ![JamesOrth](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jamesorth/32/153_2.png) [@JamesOrth](https://forum.microlist.org/u/JamesOrth)\
**Post date:** [January 7, 2020, 3:51pm UTC](https://forum.microlist.org/t/transwell-migration-imaging/732/2 "2020-01-07T15:51:25Z")

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This sounds tricky given the typical orientation of a transwell device. Depending on the size of the device and material the cells are migrating through, it may be possible to use a long working distance objective and perform z-series through the thickness of the well, as long as there isn’t too much autofluorescence of the material the cells are migrating through. This sounds like a fun thing to try, I hope it works for you.