# TIRF laser power (Nikon Ti2 + Manual TIRF module) **URL:** \ **Category:** Hardware\ **Created:** [October 6, 2023, 7:52am UTC](https://forum.microlist.org/t/tirf-laser-power-nikon-ti2-manual-tirf-module/2289 "2023-10-06T07:52:12Z")\ **Posts on this page:** 2\ **Page:** 1
**Author:** ![smson](https://avatars.discourse-cdn.com/v4/letter/s/90ced4/32.png) [@smson](https://forum.microlist.org/u/smson)\ **Post date:** [October 6, 2023, 7:52am UTC](https://forum.microlist.org/t/tirf-laser-power-nikon-ti2-manual-tirf-module/2289/1 "2023-10-06T07:52:12Z")
Hello, I’m setting up Nikon Ti2 with a manual TIRF module. I’m using LU-N4 laser launch equipped with four laser lines. The powers at the fiber output are 15-20mW for all four lasers (at 100% setting), but they are only ~0.5mW at the sample plane. Is this level of loss normal for the manual TIRF moddule+filter cube? Or is it possible I configured the system wrong? The light out of an objective seems well-collimated. Thanks! Sungmin ---
**Author:** ![Hazen\_Babcock](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/hazen_babcock/32/281_2.png) [@Hazen\_Babcock](https://forum.microlist.org/u/Hazen_Babcock)\ **Post date:** [October 16, 2023, 6:42pm UTC](https://forum.microlist.org/t/tirf-laser-power-nikon-ti2-manual-tirf-module/2289/2 "2023-10-16T18:42:14Z")
Hello, If you haven’t already solved this… Can you describe the setup a bit more? The fiber from the LU-N4 is connected directly into the manual TIRF module? The manual TIRF module is a Nikon product or something that you put together? I would not be surprised by that you’d lose 1/2-2/3 of the power between the fiber and what comes out of the objective, however 20x does seem a bit large. How much power to you measure going into the objective? You could measure this by removing the objective or rotating the turret to a position that doesn’t have an objective. best, -Hazen