# Several minute delay before acquisition starts

**URL:** <https://forum.microlist.org/t/several-minute-delay-before-acquisition-starts/2356>\
**Category:** Software\
**Tags:** micro-manager\
**Created:** [December 19, 2023, 5:02pm UTC](https://forum.microlist.org/t/several-minute-delay-before-acquisition-starts/2356 "2023-12-19T17:02:50Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Yossi\_Steinberger](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/yossi_steinberger/32/1272_2.png) [@Yossi\_Steinberger](https://forum.microlist.org/u/Yossi_Steinberger)\
**Post date:** [December 19, 2023, 5:02pm UTC](https://forum.microlist.org/t/several-minute-delay-before-acquisition-starts/2356/1 "2023-12-19T17:02:50Z")

</div>

Dear all -

I use Micromanager to acquire a time-lapse from a Sona-6 SCMOS camera, where the camera trigger is in “External Exposure” mode. I set it up to acquire 49,152 frames, where each frame is 200x200 pixels, and 12-bit, into a single image stack file of ~3.5 GB.

Strangely, there is a ~7 minute delay, after clicking “Acquire!”, until the first frame is acquired, after which the timelapse continues as expected. The delay until the first frame is only a couple of minutes if I switch the frame count value from 49,152 to ~10,000.

I checked and if I acquire this timelapse file, instead with Internal triggering, then there is no delay.

Screen shots from micromanager’s Multi-dimensional Acquisition window, and Device Property Browser window follow.

Please advise -  
Yossi

p.s., this is cross-posted on image.sc

> **[Several minute delay before acquisition starts](https://forum.image.sc/t/several-minute-delay-before-acquisition-starts/90068)**
>
> Dear all - I use Micromanager to acquire a time-lapse from a Sona-6 SCMOS camera, where the camera trigger is in “External Exposure” mode. I set it up to acquire 49,152 frames, where each frame is 200x200 pixels, and 12-bit, into a single image...

 ![image](https://us1.discourse-cdn.com/flex020/uploads/microlist/original/2X/4/405289660c1403dcdd5749cb9df224a8950e8847.png)

 ![image (1)](https://us1.discourse-cdn.com/flex020/uploads/microlist/original/2X/9/9ebe8678cc31ae4f4b2a65dde2b0237048dddd9a.png)
