# Resolution too low - advice for image aquisition needed

**URL:** <https://forum.microlist.org/t/resolution-too-low-advice-for-image-aquisition-needed/2477>\
**Category:** Hardware\
**Created:** [April 4, 2024, 3:49pm UTC](https://forum.microlist.org/t/resolution-too-low-advice-for-image-aquisition-needed/2477 "2024-04-04T15:49:48Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Hannah\_Chapman](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/hannah_chapman/32/1411_2.png) [@Hannah\_Chapman](https://forum.microlist.org/u/Hannah_Chapman)\
**Post date:** [April 4, 2024, 3:49pm UTC](https://forum.microlist.org/t/resolution-too-low-advice-for-image-aquisition-needed/2477/1 "2024-04-04T15:49:48Z")

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Hi all,

I am trying to segment fluorescent puncta. All is well on those which are far apart, but there are areas where these are very close. I am using the Zeiss Image.Z2 with Apotome.2 100x objective. Puncta should be around 200um.

As you can see in the image below, these are difficult to distinguish. I am by no means a microscopy expert, but I wonder if this is because the resolution is too low? I am wondering which system it may be best to try from here on.

Thank you in advance. Hannah.

 ![Example_annotated](https://us1.discourse-cdn.com/flex020/uploads/microlist/original/2X/6/68cb02f2ca57cad21c028631dc7f432241002471.jpeg)

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**Author:** ![dmahecic](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/dmahecic/32/1197_2.png) [@dmahecic](https://forum.microlist.org/u/dmahecic)\
**Post date:** [April 4, 2024, 5:43pm UTC](https://forum.microlist.org/t/resolution-too-low-advice-for-image-aquisition-needed/2477/2 "2024-04-04T17:43:07Z")

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Hi Hannah,

Could you clarify a bit more about which system you’re using - is it the Zeiss Axio Imager? What camera are you using?

Also, did you mean 200 um or 200 nm?

Thanks,  
Dora