# Re-usable live cell chamber

**URL:** <https://forum.microlist.org/t/re-usable-live-cell-chamber/1143>\
**Category:** Probes & Prep\
**Created:** [September 18, 2020, 4:23pm UTC](https://forum.microlist.org/t/re-usable-live-cell-chamber/1143 "2020-09-18T16:23:03Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![WillGiang](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/willgiang/32/1224_2.png) [@WillGiang](https://forum.microlist.org/u/WillGiang)\
**Post date:** [September 18, 2020, 4:23pm UTC](https://forum.microlist.org/t/re-usable-live-cell-chamber/1143/1 "2020-09-18T16:23:03Z")

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Hi,

Do people have a favorite re-usable live cell chamber? The [Attofluor](https://www.thermofisher.com/order/catalog/product/A7816#/A7816) checks all of my boxes, but I’m wondering what else is out there.

Thanks,  
Will

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**Author:** ![LPUoO](https://avatars.discourse-cdn.com/v4/letter/l/9fc348/32.png) [@LPUoO](https://forum.microlist.org/u/LPUoO)\
**Post date:** [September 23, 2020, 8:07am UTC](https://forum.microlist.org/t/re-usable-live-cell-chamber/1143/2 "2020-09-23T08:07:37Z")

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I used the Attofluor™ Cell Chamber, but I found that if one is not careful it is possible to break the coverslip if it is too tight. Otherwise it is fine.

If the cost of the Attofluor is an issue you could consider 3D printing coverslip holders. See:

> <https://twitter.com/singmolsci/status/715731422992936961>

I for example made a coverslip holder that I can connect to a water bath for temperature control and it fits on a slide holder

 ![image](https://us1.discourse-cdn.com/flex020/uploads/microlist/original/1X/e0101ca493b04975ea4406986bf92b0c6df14b2d.jpeg)  
It worked quite well.

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**Author:** ![JASlotman](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jaslotman/32/331_2.png) [@JASlotman](https://forum.microlist.org/u/JASlotman)\
**Post date:** [September 24, 2020, 11:40am UTC](https://forum.microlist.org/t/re-usable-live-cell-chamber/1143/3 "2020-09-24T11:40:25Z")

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I second the Attofluor cell, we use them in our facility all the time. When you keep good care of them the breaking of coverslips and leakage are not an issue at all.

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**Author:** ![lgelman](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/lgelman/32/303_2.png) [@lgelman](https://forum.microlist.org/u/lgelman)\
**Post date:** [September 25, 2020, 12:00pm UTC](https://forum.microlist.org/t/re-usable-live-cell-chamber/1143/4 "2020-09-25T12:00:43Z")

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Hi Will,  
You can check there as well:

> **[LIFE IMAGING SERVICES: Microscopy Chamber](http://www.lis.ch/thechamber.html)**

  
Best regards,  
Laurent.

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**Author:** ![Jaroslav](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jaroslav/32/751_2.png) [@Jaroslav](https://forum.microlist.org/u/Jaroslav)\
**Post date:** [April 1, 2021, 9:24am UTC](https://forum.microlist.org/t/re-usable-live-cell-chamber/1143/5 "2021-04-01T09:24:41Z")

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Hi, if you care about very precise temperature control, you can try the VAHEAT device with a small reservoir for medium on top of it. [VAHEAT - Micro heating system](https://interherence.com/vaheat/)

 ![vaheat](https://us1.discourse-cdn.com/flex020/uploads/microlist/original/1X/03dd1be7e2dd70e9a56a7fbd351f082714850a4c.jpeg)  
Full disclosure, I have just started working for Interherence now. I encourage you to judge it for yourself on the web and request an on site demo through the company website.