# mTurquoise2, mNeonGreen and mCherry in Yeast

**URL:** <https://forum.microlist.org/t/mturquoise2-mneongreen-and-mcherry-in-yeast/704>\
**Category:** Probes & Prep\
**Tags:** fluorescent-proteins\
**Created:** [November 12, 2019, 8:31am UTC](https://forum.microlist.org/t/mturquoise2-mneongreen-and-mcherry-in-yeast/704 "2019-11-12T08:31:17Z")\
**Posts on this page:** 6\
**Page:** 1

<div class="post-metadata">

**Author:** ![lgelman](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/lgelman/32/303_2.png) [@lgelman](https://forum.microlist.org/u/lgelman)\
**Post date:** [November 12, 2019, 8:31am UTC](https://forum.microlist.org/t/mturquoise2-mneongreen-and-mcherry-in-yeast/704/1 "2019-11-12T08:31:17Z")

</div>

Hi everybody,  
Has anyone ever successfully expressed mTurquoise2, mNeonGreen and mCherry **in Yeast**? Was there a lot of codon optimization? Any reason why we should not go for these tags in our future yeast experiments?  
Thanks and best regards,  
Laurent.

---

<div class="post-metadata">

**Author:** ![talley](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/talley/32/3_2.png) [@talley](https://forum.microlist.org/u/talley)\
**Post date:** [November 12, 2019, 12:37pm UTC](https://forum.microlist.org/t/mturquoise2-mneongreen-and-mcherry-in-yeast/704/2 "2019-11-12T12:37:35Z")

</div>

Have you seen this article?

[https://www.nature.com/articles/s41598-019-38913-z](https://www.nature.com/articles/s41598-019-38913-z)

Hard to find a better characterization than that.

---

<div class="post-metadata">

**Author:** ![lgelman](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/lgelman/32/303_2.png) [@lgelman](https://forum.microlist.org/u/lgelman)\
**Post date:** [November 12, 2019, 1:29pm UTC](https://forum.microlist.org/t/mturquoise2-mneongreen-and-mcherry-in-yeast/704/3 "2019-11-12T13:29:58Z")

</div>

Hi Talley,

Thanks a lot, I had totally forgot about this one!

Indeed, it will be difficult to do better  
J

Very best regards,

Laurent.

---

<div class="post-metadata">

**Author:** ![joachimgoedhart](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/joachimgoedhart/32/26_2.png) [@joachimgoedhart](https://forum.microlist.org/u/joachimgoedhart)\
**Post date:** [November 12, 2019, 7:21pm UTC](https://forum.microlist.org/t/mturquoise2-mneongreen-and-mcherry-in-yeast/704/4 "2019-11-12T19:21:37Z")

</div>

I was involved in that study - let me know if you have questions!

---

<div class="post-metadata">

**Author:** ![lgelman](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/lgelman/32/303_2.png) [@lgelman](https://forum.microlist.org/u/lgelman)\
**Post date:** [November 13, 2019, 7:38am UTC](https://forum.microlist.org/t/mturquoise2-mneongreen-and-mcherry-in-yeast/704/5 "2019-11-13T07:38:48Z")

</div>

Hi Joachim,

Thanks, I’ll do!

Best,

Laurent.

---

<div class="post-metadata">

**Author:** ![JamesOrth](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jamesorth/32/153_2.png) [@JamesOrth](https://forum.microlist.org/u/JamesOrth)\
**Post date:** [January 7, 2020, 4:10pm UTC](https://forum.microlist.org/t/mturquoise2-mneongreen-and-mcherry-in-yeast/704/6 "2020-01-07T16:10:52Z")

</div>

Definitely have imaged Cherry for a user, it was dim-ish and liked to bleach, but played with imaging conditions and was able to image about 20 minutes or a bit longer with no photobleaching. I have no experience with the others in yeast. Good luck! BTW - mRuby seemed better in yeast than Cherry.