# Mineral and silicone oil for Lightsheet microscopy **URL:** \ **Category:** Probes & Prep\ **Tags:** light-sheet\ **Created:** [January 23, 2020, 10:49am UTC](https://forum.microlist.org/t/mineral-and-silicone-oil-for-lightsheet-microscopy/822 "2020-01-23T10:49:39Z")\ **Posts on this page:** 3\ **Page:** 1
**Author:** ![Alex.h](https://avatars.discourse-cdn.com/v4/letter/a/dec6dc/32.png) [@Alex.h](https://forum.microlist.org/u/Alex.h)\ **Post date:** [January 23, 2020, 10:49am UTC](https://forum.microlist.org/t/mineral-and-silicone-oil-for-lightsheet-microscopy/822/1 "2020-01-23T10:49:39Z")
Dear all, We are looking to use oil for refractive index matching with the new version of the CUBIC (CUBIC-L and CUBIC -R). We need large quantities (30ml of mililiters for each run). The final RI will be 1.52 Does anybody have sources to order silicone and mineral oil for Europe ? And do you have some advice, how to wash the chamber of the lightsheet, how to mix the different oil and if they are stable etc… I found from a paper Shin Etsu silicones oil HVAC F-4 RI 1.555 (US compagny) mineral oil (RI = 1.467,M8410,Sigma-Aldrich ok for EU) ---
**Author:** ![nvladimus](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/nvladimus/32/285_2.png) [@nvladimus](https://forum.microlist.org/u/nvladimus)\ **Post date:** [January 24, 2020, 2:55pm UTC](https://forum.microlist.org/t/mineral-and-silicone-oil-for-lightsheet-microscopy/822/2 "2020-01-24T14:55:57Z")
[https://www.cargille.com/immersion-oils/](https://www.cargille.com/immersion-oils/) is a popular choice for low-cost immersion oils in microscopy. ---
**Author:** ![Alex.h](https://avatars.discourse-cdn.com/v4/letter/a/dec6dc/32.png) [@Alex.h](https://forum.microlist.org/u/Alex.h)\ **Post date:** [January 27, 2020, 11:00am UTC](https://forum.microlist.org/t/mineral-and-silicone-oil-for-lightsheet-microscopy/822/3 "2020-01-27T11:00:51Z")
Do how know if it’s better to buy an unique oil at 1.51 or if I really need to mix silicones and mineral oil?