# Measure diameter of microbubble images

**URL:** <https://forum.microlist.org/t/measure-diameter-of-microbubble-images/753>\
**Category:** Probes & Prep\
**Created:** [December 6, 2019, 12:34am UTC](https://forum.microlist.org/t/measure-diameter-of-microbubble-images/753 "2019-12-06T00:34:56Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![Jamessmp23](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jamessmp23/32/465_2.png) [@Jamessmp23](https://forum.microlist.org/u/Jamessmp23)\
**Post date:** [December 6, 2019, 12:34am UTC](https://forum.microlist.org/t/measure-diameter-of-microbubble-images/753/1 "2019-12-06T00:34:56Z")

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Hi everyone, I’m trying to measure diameter of microbubble images but I got a difficulty in image threshold. the gray color is so close to the bubble color even the reflection inside bubbles.  
I try to measure it one by one and it takes lots of time. Any solution or suggestion please share 🙂  
PS. I used a micro lens camera with backlight technique to take this microbubbles pictures if anyone have suggestions on camera works also please guide me.

 ![riser 1000](https://us1.discourse-cdn.com/flex020/uploads/microlist/original/1X/d70dcb56f7a7ecb66078820570676ad49e22798d.jpeg)

Thank you 🙂  
James

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**Author:** ![Hazen\_Babcock](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/hazen_babcock/32/281_2.png) [@Hazen\_Babcock](https://forum.microlist.org/u/Hazen_Babcock)\
**Post date:** [December 6, 2019, 9:40pm UTC](https://forum.microlist.org/t/measure-diameter-of-microbubble-images/753/2 "2019-12-06T21:40:27Z")

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Do you have any control over the sample density? I imagine that it would be a lot easier if there were fewer microbubbles.

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**Author:** ![Jamessmp23](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jamessmp23/32/465_2.png) [@Jamessmp23](https://forum.microlist.org/u/Jamessmp23)\
**Post date:** [December 8, 2019, 8:29am UTC](https://forum.microlist.org/t/measure-diameter-of-microbubble-images/753/3 "2019-12-08T08:29:50Z")

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Thanks for the reply,  
I need to fixed the air flowrate in this condition, even the low flowrate (100ml/min) also have this problem.

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**Author:** ![Hazen\_Babcock](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/hazen_babcock/32/281_2.png) [@Hazen\_Babcock](https://forum.microlist.org/u/Hazen_Babcock)\
**Post date:** [December 8, 2019, 4:19pm UTC](https://forum.microlist.org/t/measure-diameter-of-microbubble-images/753/4 "2019-12-08T16:19:16Z")

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Maybe you could provide a diagram showing how you are currently doing the imaging? Also, I’m not sure what “micro lens camera” is. Is this a camera with lens on the chip to improve quantum efficiency? Or are you trying to do some kind of light field imaging with a micro lens array?

Assuming that you are imaging with a normal upright or inverted microscope geometry I’d suggest dark field imaging as a possible alternative. Then hopefully you would only see the light scattered by the bubbles, and they would appear as bright spots on a dark background. You might also be able to figure out a relation between the brightness and the size after analyzing a few examples by hand, and then you could use this relation to quickly estimate the size of all of the bubbles in the FOV.

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**Author:** ![JamesOrth](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jamesorth/32/153_2.png) [@JamesOrth](https://forum.microlist.org/u/JamesOrth)\
**Post date:** [January 7, 2020, 3:46pm UTC](https://forum.microlist.org/t/measure-diameter-of-microbubble-images/753/5 "2020-01-07T15:46:31Z")

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Can you trap a fluorescent carrier inside the microbubbles when they are produced? Presumably this would provide massive signal to noise as long as your camera is for imaging fluorescence.