To combat problems with phototoxicity, I’ve had luck using free radical scavengers in the media in the past (Trolox, Ascorbic Acid and Catalase).
I don’t think we ever did a head-to-head on how much of a difference it made, but you might find it worth trying.
Hi there, my first question in this super helpful forum.
In our lab we do long-term (72-96 hs) live microscopy of yeast cells grown in microfluidic chips, at 30ºC. Using a 40x air objective I usually monitor 50 different positions every 10 minutes.
I’d like to improve resolution moving to an immersion objective, but which one?
Oil? Does it get gummy, or dries?
Silicone Oil?
Water with a dispenser? (sounds expensive and tricky to setup)
Thanks you all in advance,
Pablo