# How can I improve image stitching with the AxioScan 7?

**URL:** https://forum.microlist.org/t/how-can-i-improve-image-stitching-with-the-axioscan-7/3257
**Category:** Software
**Created:** [October 21, 2025, 7:09pm UTC](https://forum.microlist.org/t/how-can-i-improve-image-stitching-with-the-axioscan-7/3257 "2025-10-21T19:09:46Z")
**Posts on this page:** 1
**Showing post:** 2

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### Author: ![rlokkie](https://avatars.discourse-cdn.com/v4/letter/r/a9adbd/32.png) [@rlokkie](https://forum.microlist.org/u/rlokkie)
#### Post date: [October 22, 2025, 10:14am UTC](https://forum.microlist.org/t/how-can-i-improve-image-stitching-with-the-axioscan-7/3257/2 "2025-10-22T10:14:31Z")

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Looks like the red channel is somewhat diffuse staining, it could be that the low contrast leads to the stitching issues.

If I recall correctly the stitching is done on the first channel imaged (or the focus reference?), perhaps changing the acquisition order dapi\>whatever is in red will already lead to better results (don’t quote me on this one), when using '“online” stitching (default).

There is also the ‘‘offline’’ and ‘‘fuse active’’ options under ‘online-processing’ that may improve the stitching results.

You can also restitch post-acquisition with zen, and then only select the channel with good/best contrast.  
There is some additional info on post-acquisition stitching in the post below:

> [@Stitching of tile images Axio imager/Zen blue](https://forum.microlist.org/t/stitching-of-tile-images-axio-imager-zen-blue/2172/2):
>
> A thing that has always bothered me about Zen blue is that the max stitching overlap is the same as the approximate acquisition overlap (10%). Maybe increasing the max shift to ~12% or so will help?

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