# How can I improve image stitching with the AxioScan 7?
**URL:** https://forum.microlist.org/t/how-can-i-improve-image-stitching-with-the-axioscan-7/3257
**Category:** Software
**Created:** [October 21, 2025, 7:09pm UTC](https://forum.microlist.org/t/how-can-i-improve-image-stitching-with-the-axioscan-7/3257 "2025-10-21T19:09:46Z")
**Posts on this page:** 3
**Page:** 1
### Author:  [@danlamon](https://forum.microlist.org/u/danlamon)
#### Post date: [October 21, 2025, 7:09pm UTC](https://forum.microlist.org/t/how-can-i-improve-image-stitching-with-the-axioscan-7/3257/1 "2025-10-21T19:09:46Z")
Hi all,
I have some 20x images acquired with AxioScan 7 that are showing breaks in the overlap. The stitches are set at 20% at the moment.
What’s the best approach to improving the stitching? Is it reasonable to expect better raw images from our imaging centre, or is this normal and should be improved using post-hoc touch-ups and plug-ins?
Thanks in advance.
---
### Author:  [@rlokkie](https://forum.microlist.org/u/rlokkie)
#### Post date: [October 22, 2025, 10:14am UTC](https://forum.microlist.org/t/how-can-i-improve-image-stitching-with-the-axioscan-7/3257/2 "2025-10-22T10:14:31Z")
Looks like the red channel is somewhat diffuse staining, it could be that the low contrast leads to the stitching issues.
If I recall correctly the stitching is done on the first channel imaged (or the focus reference?), perhaps changing the acquisition order dapi\>whatever is in red will already lead to better results (don’t quote me on this one), when using '“online” stitching (default).
There is also the ‘‘offline’’ and ‘‘fuse active’’ options under ‘online-processing’ that may improve the stitching results.
You can also restitch post-acquisition with zen, and then only select the channel with good/best contrast.
There is some additional info on post-acquisition stitching in the post below:
> [@Stitching of tile images Axio imager/Zen blue](https://forum.microlist.org/t/stitching-of-tile-images-axio-imager-zen-blue/2172/2):
>
> A thing that has always bothered me about Zen blue is that the max stitching overlap is the same as the approximate acquisition overlap (10%). Maybe increasing the max shift to ~12% or so will help?
---
### Author:  [@danlamon](https://forum.microlist.org/u/danlamon)
#### Post date: [October 22, 2025, 6:09pm UTC](https://forum.microlist.org/t/how-can-i-improve-image-stitching-with-the-axioscan-7/3257/3 "2025-10-22T18:09:42Z")
I’ll look into those options! And thank you for linking the thread that solved this issue earlier, I must have missed it when browsing the forum. I really appreciate it!