# Homemade Mounting Media

**URL:** <https://forum.microlist.org/t/homemade-mounting-media/908>\
**Category:** Probes & Prep\
**Created:** [March 5, 2020, 10:54pm UTC](https://forum.microlist.org/t/homemade-mounting-media/908 "2020-03-05T22:54:28Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![EricGriffis](https://avatars.discourse-cdn.com/v4/letter/e/58f4c7/32.png) [@EricGriffis](https://forum.microlist.org/u/EricGriffis)\
**Post date:** [March 5, 2020, 10:54pm UTC](https://forum.microlist.org/t/homemade-mounting-media/908/1 "2020-03-05T22:54:28Z")

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Does anyone have a mounting media that they love for multi-well plates? We’ve been recommending the standard 75% glycerol + propyl gallate, but if there is a better one, we’d be happy to try it. Bonus points if it has a refractive index low enough to allow reflection-based autofocus to work.

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**Author:** ![GopherConfocal](https://avatars.discourse-cdn.com/v4/letter/g/2bfe46/32.png) [@GopherConfocal](https://forum.microlist.org/u/GopherConfocal)\
**Post date:** [March 11, 2020, 12:33pm UTC](https://forum.microlist.org/t/homemade-mounting-media/908/2 "2020-03-11T12:33:13Z")

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I used a very similar mounting medium in the past: 75% glycerol/4% n-propyl gallate/25 mM Tris, pH 8.0.