# Heat shock induction in petri dish

**URL:** <https://forum.microlist.org/t/heat-shock-induction-in-petri-dish/1899>\
**Category:** Hardware\
**Tags:** confocal\
**Created:** [August 24, 2022, 2:11pm UTC](https://forum.microlist.org/t/heat-shock-induction-in-petri-dish/1899 "2022-08-24T14:11:06Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![Norbert\_Bollier](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/norbert_bollier/32/1036_2.png) [@Norbert\_Bollier](https://forum.microlist.org/u/Norbert_Bollier)\
**Post date:** [August 24, 2022, 2:11pm UTC](https://forum.microlist.org/t/heat-shock-induction-in-petri-dish/1899/1 "2022-08-24T14:11:06Z")

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Hello everybody,

I’m looking for an efficient system to induce heat shock (45 degrees) on plant organs (meristems) placed in petri dishes and observed under an upright confocal microscope. I need to image whilde inducing the heat shock (from RT to 45°C, not necessary very fast)

In practice the organs are inserted in agarose 1% in in a petri dish and covered by liquid culture medium, the imaging is done using an immersion objective.

I’m wondering if it would be efficient to use a perfusion system combined with a temperature controller to change the temperature or if a petri dish heater would be sufficient?  
Does anybody have ever used this kind of system: [Open heated perfusion chamber for Water Immersion Objectives of upright and long working distance objectives of inverted microscopes, TC-WI, temperature controlled microscope stages](http://biosciencetools.com/catalog/Heated_WaterImmersion.htm) with a temperature controller for example to do a heat shock while imaging.

Thanks a lot in advance for you tips or suggestions,

Best,

Norbert