# FLIM: Easy sample for product demonstrations?

**URL:** <https://forum.microlist.org/t/flim-easy-sample-for-product-demonstrations/3172>\
**Category:** Probes & Prep\
**Created:** [July 19, 2025, 12:04am UTC](https://forum.microlist.org/t/flim-easy-sample-for-product-demonstrations/3172 "2025-07-19T00:04:24Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![phoTONIcs](https://avatars.discourse-cdn.com/v4/letter/p/45deac/32.png) [@phoTONIcs](https://forum.microlist.org/u/phoTONIcs)\
**Post date:** [July 19, 2025, 12:04am UTC](https://forum.microlist.org/t/flim-easy-sample-for-product-demonstrations/3172/1 "2025-07-19T00:04:24Z")

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Hello Micro-community!  
Is there an “easy to prep” (non-labor intensive/few reagents) FLIM sample to use to train microscopy users? Thats visually impressive, but gets the job done?

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**Author:** ![llamero](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/llamero/32/716_2.png) [@llamero](https://forum.microlist.org/u/llamero)\
**Post date:** [July 19, 2025, 12:51am UTC](https://forum.microlist.org/t/flim-easy-sample-for-product-demonstrations/3172/2 "2025-07-19T00:51:03Z")

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If it is for 2P FLIM I would recommend 4-methyl-2-nitroaniline. Suspend the cystals in permount, and you’ll have both a permanent demo slide and calibration slide. It has highly efficient second harmonic generation, so the lifetime is 0 ns which is great for calibrating FLIM. It is also visually cool as you can clearly see the SHG light by eye.

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**Author:** ![phoTONIcs](https://avatars.discourse-cdn.com/v4/letter/p/45deac/32.png) [@phoTONIcs](https://forum.microlist.org/u/phoTONIcs)\
**Post date:** [July 19, 2025, 1:06am UTC](https://forum.microlist.org/t/flim-easy-sample-for-product-demonstrations/3172/3 "2025-07-19T01:06:44Z")

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thanks! its for 1P FLIM, but i appreciate the idea!

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**Author:** ![TEFK](https://avatars.discourse-cdn.com/v4/letter/t/3bc359/32.png) [@TEFK](https://forum.microlist.org/u/TEFK)\
**Post date:** [July 24, 2025, 8:09am UTC](https://forum.microlist.org/t/flim-easy-sample-for-product-demonstrations/3172/4 "2025-07-24T08:09:24Z")

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Hi,  
A very practical sample might be pollen grains for example from the plant Convallaria. Such samples are not expensive and are robust.

Best,  
TEFK