# Filtersets for NIR SMLM dyes

**URL:** <https://forum.microlist.org/t/filtersets-for-nir-smlm-dyes/1198>\
**Category:** Uncategorized\
**Created:** [November 11, 2020, 1:47pm UTC](https://forum.microlist.org/t/filtersets-for-nir-smlm-dyes/1198 "2020-11-11T13:47:27Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![A.P\_Wheeler](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/a.p_wheeler/32/98_2.png) [@A.P\_Wheeler](https://forum.microlist.org/u/A.P_Wheeler)\
**Post date:** [November 11, 2020, 1:47pm UTC](https://forum.microlist.org/t/filtersets-for-nir-smlm-dyes/1198/1 "2020-11-11T13:47:27Z")

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We are upgrading our SMLM system and I’m interested in getting a filterset to allow us to do SMLM with NIR dyes, e.g. AF700, Cy5.5 etc. Obviously don’t want any bleedthrough from AF647 - which is our goto SMLM dye, since the aim would be to multiplex. Does anyone have any experience with good single or multiple bandpass filters for this?

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**Author:** ![Hazen\_Babcock](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/hazen_babcock/32/281_2.png) [@Hazen\_Babcock](https://forum.microlist.org/u/Hazen_Babcock)\
**Post date:** [November 27, 2020, 9:15pm UTC](https://forum.microlist.org/t/filtersets-for-nir-smlm-dyes/1198/2 "2020-11-27T21:15:09Z")

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I have used the following from Chroma:

Emitter:  
Supplier Item: zet405/488/561/647-656/75 Custom multi-band laser emission filter, 25mm diameter.

Dichroic:  
zt405/488/561/647/752rpc Polychroic laser mirror, 26x38x2mm thick.

These are 5 band filters (405, 488, 561, 649, 750) and so are really expensive. You might talk to Chroma as they are usually willing to make you whatever you want for a similar price to what they typically stock.

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**Author:** ![Hazen\_Babcock](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/hazen_babcock/32/281_2.png) [@Hazen\_Babcock](https://forum.microlist.org/u/Hazen_Babcock)\
**Post date:** [November 27, 2020, 9:18pm UTC](https://forum.microlist.org/t/filtersets-for-nir-smlm-dyes/1198/3 "2020-11-27T21:18:08Z")

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Looks like you want something that is a little shorter in NIR wavelength. I’d guess this won’t be easy as A647 still has substantial emission past 700. You might have better luck with 647 excitation and ratiometric detection.

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**Author:** ![A.P\_Wheeler](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/a.p_wheeler/32/98_2.png) [@A.P\_Wheeler](https://forum.microlist.org/u/A.P_Wheeler)\
**Post date:** [December 15, 2020, 4:18pm UTC](https://forum.microlist.org/t/filtersets-for-nir-smlm-dyes/1198/4 "2020-12-15T16:18:27Z")

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Hi Hazen sorry for the slow reply - we are currently upgrading all the super-res equipment in our facility and I got sidetracked. The emitter and Dichroic look lilke really good option for us thanks for the suggestion. I think our end users will not be too fussy. However they are very ambitious and are already discussing 3 colour STORM. I realistically think this will be ‘orange’ - perhaps Janeila Fluor 549, I can get AF555 to blink a bit if not, AF647 as our favourite and then something 750 ish. We already have people trying to get 3 colour DNA PAINT going who will be delighted to add a 4th fluor as well. Much appreciated.