# Dorsal skin flap protocol suggestions

**URL:** <https://forum.microlist.org/t/dorsal-skin-flap-protocol-suggestions/1424>\
**Category:** Probes & Prep\
**Created:** [May 29, 2021, 4:06am UTC](https://forum.microlist.org/t/dorsal-skin-flap-protocol-suggestions/1424 "2021-05-29T04:06:20Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![ShawnPatrickCasey](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/shawnpatrickcasey/32/712_2.png) [@ShawnPatrickCasey](https://forum.microlist.org/u/ShawnPatrickCasey)\
**Post date:** [May 29, 2021, 4:06am UTC](https://forum.microlist.org/t/dorsal-skin-flap-protocol-suggestions/1424/1 "2021-05-29T04:06:20Z")

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One of our shared labs has been loaned a 2P version of [IVIM Technology – Global No. 1 solution provider of IntraVital Microscopy](http://ivimtech.com/?ckattempt=1) with a Coherent Axon 920. They have 4 minute promotional video showing cranial and dorsal skin flap surgery followed by imaging. So, I have some limited experience with the cranial window and realize its a marketing video, in fast forward. Does anyone have a suggestion for a detailed dorsal flap window surgical protocol that they have tried? With photos or a JOVE type video? Trying to help those in the shared lab to better evaluate the system while it is on loan.

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**Author:** ![JamesOrth](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jamesorth/32/153_2.png) [@JamesOrth](https://forum.microlist.org/u/JamesOrth)\
**Post date:** [May 31, 2021, 4:48pm UTC](https://forum.microlist.org/t/dorsal-skin-flap-protocol-suggestions/1424/2 "2021-05-31T16:48:30Z")

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Hi Shawn -

I believe there was something published in JoVE that may show something?  
Intravital Microscopy of Tumor-associated Vasculature Using Advanced

Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice

11 Ann L.B. Seynhaeve , Timo L.M. ten Hagen

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**Author:** ![ShawnPatrickCasey](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/shawnpatrickcasey/32/712_2.png) [@ShawnPatrickCasey](https://forum.microlist.org/u/ShawnPatrickCasey)\
**Post date:** [June 7, 2021, 12:32pm UTC](https://forum.microlist.org/t/dorsal-skin-flap-protocol-suggestions/1424/3 "2021-06-07T12:32:09Z")

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Yes, this is the sort of thing. Other suggestions/videos/webinar would be appreciated.
