# Does airyscan detector rearrangement affect intensity? 

**URL:** <https://forum.microlist.org/t/does-airyscan-detector-rearrangement-affect-intensity/2818>\
**Category:** Hardware\
**Tags:** airyscan\
**Created:** [September 30, 2024, 2:21pm UTC](https://forum.microlist.org/t/does-airyscan-detector-rearrangement-affect-intensity/2818 "2024-09-30T14:21:53Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![ejrwls0130](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/ejrwls0130/32/1581_2.png) [@ejrwls0130](https://forum.microlist.org/u/ejrwls0130)\
**Post date:** [September 30, 2024, 2:21pm UTC](https://forum.microlist.org/t/does-airyscan-detector-rearrangement-affect-intensity/2818/1 "2024-09-30T14:21:53Z")

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I have just small question.

I usually use the LSM980 Airyscan for imaging cells.

When I change plates to image a different one, the Airyscan detector starts rearranging its position, and I can see a change in brightness.

(rearrangement means - airy scan detector adjustment: fiber positon)

I wonder if this process affects the intensity values of the samples?

I want to compare the relative intensity values between different plates.

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**Author:** ![jasonkirk](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jasonkirk/32/668_2.png) [@jasonkirk](https://forum.microlist.org/u/jasonkirk)\
**Post date:** [October 1, 2024, 4:45pm UTC](https://forum.microlist.org/t/does-airyscan-detector-rearrangement-affect-intensity/2818/2 "2024-10-01T16:45:10Z")

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It will. You need to realign the array each time you change your sample.

The coverglass bottoms of multi-well plates can be horribly unreliable with respect to thickness - and unless you are using high precision #1.5 (170um) plates you will notice quite a bit of variability in the alignment. Changing to high precision plates will help.

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**Author:** ![ejrwls0130](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/ejrwls0130/32/1581_2.png) [@ejrwls0130](https://forum.microlist.org/u/ejrwls0130)\
**Post date:** [October 11, 2024, 6:22am UTC](https://forum.microlist.org/t/does-airyscan-detector-rearrangement-affect-intensity/2818/3 "2024-10-11T06:22:45Z")

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Thanks for your reply.

Then how can I compare the intensity at different plate.

If Airyscan detector realign affect intensity, it seems hard to comparing samples at different plate.

Do I have to use just confocal mode without airyscan?