# Culture cell medium influence on fluorescence or SHG and THG

**URL:** https://forum.microlist.org/t/culture-cell-medium-influence-on-fluorescence-or-shg-and-thg/928
**Category:** Uncategorized
**Created:** [April 3, 2020, 11:25am UTC](https://forum.microlist.org/t/culture-cell-medium-influence-on-fluorescence-or-shg-and-thg/928 "2020-04-03T11:25:16Z")
**Posts on this page:** 2
**Page:** 1

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### Author: ![Alex.h](https://avatars.discourse-cdn.com/v4/letter/a/dec6dc/32.png) [@Alex.h](https://forum.microlist.org/u/Alex.h)
#### Post date: [April 3, 2020, 11:25am UTC](https://forum.microlist.org/t/culture-cell-medium-influence-on-fluorescence-or-shg-and-thg/928/1 "2020-04-03T11:25:16Z")

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Hello everybodies,  
Do you know if medium or phenol red can have an influence on fluorescence or second harmonic or third harmonic generation??

kind regards

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### Author: ![Oleg](https://avatars.discourse-cdn.com/v4/letter/o/ebca7d/32.png) [@Oleg](https://forum.microlist.org/u/Oleg)
#### Post date: [April 18, 2020, 2:29am UTC](https://forum.microlist.org/t/culture-cell-medium-influence-on-fluorescence-or-shg-and-thg/928/2 "2020-04-18T02:29:53Z")

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Hi @Alex.h,

I never did SHG/THG, but as for fluorescence - yes, media might have an influence, but it depends… For conventional DMEM w/ phenol red I never have an issue to track a cell cycle progression using mCherry fluorescence as a reporter. But in this case I didn’t care much about precision. However, for the FRET measurement with CFP/YFP pair I switched to FluoroBrite DMEM (proprietary media from ThermoFisher) because DMEM, even phenol red free, gave me too much background.

Maybe you already found the answer for SHG/THG elsewhere, if so, please share)

Best,  
Oleg