I read some article, and I found a lot of different protocol for the clearing.
We already use some protocol in our platform with a lightsheet Z1 from Zeiss.
- X clarity for adult mice brain
- CUBIC for organoid and embryo mice brain
- RapiClear +/- RIMS for small samples (with +/- result)
- Reverse BABB (an organic solvent based method) for lungs. Careful organic solvent properties can be very corrosive for the lens. This is why We reverse a bit the protocol.
Do you have some other protocol to share with me based on your experiment?