# Cell counting with ImageJ

**URL:** <https://forum.microlist.org/t/cell-counting-with-imagej/817>\
**Category:** Uncategorized\
**Tags:** imagej\
**Created:** [January 21, 2020, 1:27pm UTC](https://forum.microlist.org/t/cell-counting-with-imagej/817 "2020-01-21T13:27:07Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Katie\_Lui](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/katie_lui/32/482_2.png) [@Katie\_Lui](https://forum.microlist.org/u/Katie_Lui)\
**Post date:** [January 21, 2020, 1:27pm UTC](https://forum.microlist.org/t/cell-counting-with-imagej/817/1 "2020-01-21T13:27:07Z")

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I was trying to do cell counting analysis on some of my cultured rat astrocytes whose boundaries are quite indistinct as the cells are flat and confluent, by watershed after having done some adjustment to the images. The main problem I think I have here is that the colour intensity of the “background” is almost the same as that in the “cell body” on the images. How should I approach it?

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**Author:** ![jennifer](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jennifer/32/634_2.png) [@jennifer](https://forum.microlist.org/u/jennifer)\
**Post date:** [January 21, 2020, 2:04pm UTC](https://forum.microlist.org/t/cell-counting-with-imagej/817/2 "2020-01-21T14:04:43Z")

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Hi Katie. This forum focuses on image acquisition. The analysis experts (including many of the ImageJ developers) are on the image.sc forum. You should ask your question there.

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**Author:** ![Katie\_Lui](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/katie_lui/32/482_2.png) [@Katie\_Lui](https://forum.microlist.org/u/Katie_Lui)\
**Post date:** [January 21, 2020, 2:21pm UTC](https://forum.microlist.org/t/cell-counting-with-imagej/817/4 "2020-01-21T14:21:39Z")

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Hi Jennifer,  
I see - thank you so much!