# Bacterial staining on formaline fixed spheroids

**URL:** <https://forum.microlist.org/t/bacterial-staining-on-formaline-fixed-spheroids/1697>\
**Category:** Probes & Prep\
**Tags:** light-sheet, fluorescent-proteins, confocal\
**Created:** [January 24, 2022, 4:09pm UTC](https://forum.microlist.org/t/bacterial-staining-on-formaline-fixed-spheroids/1697 "2022-01-24T16:09:47Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![pootaj](https://avatars.discourse-cdn.com/v4/letter/p/7ea924/32.png) [@pootaj](https://forum.microlist.org/u/pootaj)\
**Post date:** [January 24, 2022, 4:09pm UTC](https://forum.microlist.org/t/bacterial-staining-on-formaline-fixed-spheroids/1697/1 "2022-01-24T16:09:47Z")

</div>

Hi,  
I am working with human cells spheroids model. I am infecting spheroids with bacteria and using Baclight red bacterial stain for visualising bacteria in the spheroid. I have noticed that Baclight red stains bacteria and also mammalian cells which makes it difficult to differentiate. Can anyone recommend a good dye or antibody for specifically staining bacteria (gram+ve and –ve) formaldehyde-fixed human cells spheroid?  
Thanks