# 96-well plates you would recommend for high NA imaging **URL:** \ **Category:** Hardware\ **Created:** [July 25, 2022, 10:55am UTC](https://forum.microlist.org/t/96-well-plates-you-would-recommend-for-high-na-imaging/1868 "2022-07-25T10:55:18Z")\ **Posts on this page:** 8\ **Page:** 1
**Author:** ![NicoKiaru](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/nicokiaru/32/451_2.png) [@NicoKiaru](https://forum.microlist.org/u/NicoKiaru)\ **Post date:** [July 25, 2022, 10:55am UTC](https://forum.microlist.org/t/96-well-plates-you-would-recommend-for-high-na-imaging/1868/1 "2022-07-25T10:55:19Z")
Hello everybody, I’d like to know if you have some recommendations for 96-well plates (well not necessarily 96) that would be suitable for high-NA imaging. Ideally, the well plate would: - have a very homogeneous thickness, of the order 170 - 190 um - have no skirt, thus allowing to focus all wells with high-NA objectives - be reasonably cheap The well-plates we recommend at our facility is the [PhenoPlate 96](https://www.perkinelmer.com/uk/product/cellcarrier-96-ultra-lid-8x20b-6055302) from Perkin-Elmer, but we would like to know alternative options. Thanks! Nicolas (this will be crossposted on the confocal list) ---
**Author:** ![manerotoni](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/manerotoni/32/398_2.png) [@manerotoni](https://forum.microlist.org/u/manerotoni)\ **Post date:** [July 29, 2022, 8:44am UTC](https://forum.microlist.org/t/96-well-plates-you-would-recommend-for-high-na-imaging/1868/2 "2022-07-29T08:44:36Z")
Hello, we use often those [ https://zell-kontakt.de/products/imaging-plate/imaging-plate-glasscover-2/](https://zell-kontakt.de/products/imaging-plate/imaging-plate-glasscover-2/) They are fine. I normally avoid to image the outer wells as it get quite tight. It depends on how bulky your objective is. Antonio ---
**Author:** ![JamesOrth](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jamesorth/32/153_2.png) [@JamesOrth](https://forum.microlist.org/u/JamesOrth)\ **Post date:** [August 8, 2022, 8:30pm UTC](https://forum.microlist.org/t/96-well-plates-you-would-recommend-for-high-na-imaging/1868/3 "2022-08-08T20:30:46Z")
You may have had other replies, we’ve had good luck with 96 well plates from CellVis, they use 1 piece of #1.5H German glass and are well priced. They do have a skirt so you likely can’t image the entire plate with high-NA low WD lenses. ---
**Author:** ![phoTONIcs](https://avatars.discourse-cdn.com/v4/letter/p/45deac/32.png) [@phoTONIcs](https://forum.microlist.org/u/phoTONIcs)\ **Post date:** [August 8, 2022, 10:21pm UTC](https://forum.microlist.org/t/96-well-plates-you-would-recommend-for-high-na-imaging/1868/4 "2022-08-08T22:21:46Z")
[https://www.auroramicroplates.com/#products](https://www.auroramicroplates.com/#products) ---
**Author:** ![NicoKiaru](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/nicokiaru/32/451_2.png) [@NicoKiaru](https://forum.microlist.org/u/NicoKiaru)\ **Post date:** [August 23, 2022, 10:27am UTC](https://forum.microlist.org/t/96-well-plates-you-would-recommend-for-high-na-imaging/1868/5 "2022-08-23T10:27:34Z")
Hi everybody, Thank you for your answers! I’ve made a google sheet with the suggestions coming from this forum and with the ones from the confocal list: > **[A selection of 96 well plates for High NA imaging](https://docs.google.com/spreadsheets/d/1b-hA_khruxoGfJ4QfVnz98Wsb_EEOZrUn7gL7vUfm88/edit?usp=sharing)** > > Feuille 1 > > The well plates listed should be compatible with high-NA imaging, meaning: > \- have a very homogeneous bottom thickness, of the order 170 - 190 um > \- have no skirt, thus allowing to focus all wells with high-NA objectives > Brand,Link (96... If you want to edit it to add some more, please let me know and I’ll give you edit rights. Nicolas ---
**Author:** ![jdelgadozmb](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jdelgadozmb/32/1042_2.png) [@jdelgadozmb](https://forum.microlist.org/u/jdelgadozmb)\ **Post date:** [September 5, 2022, 8:57am UTC](https://forum.microlist.org/t/96-well-plates-you-would-recommend-for-high-na-imaging/1868/6 "2022-09-05T08:57:55Z")
Thanks for compiling the list! We also have good experience with Corning 4580 half area glass bottom plates if you’d like to add ---
**Author:** ![NicoKiaru](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/nicokiaru/32/451_2.png) [@NicoKiaru](https://forum.microlist.org/u/NicoKiaru)\ **Post date:** [September 5, 2022, 10:22am UTC](https://forum.microlist.org/t/96-well-plates-you-would-recommend-for-high-na-imaging/1868/7 "2022-09-05T10:22:02Z")
Thanks, I’ve added it, but I couldn’t find the skirt size unfortunately ---
**Author:** ![jdelgadozmb](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/jdelgadozmb/32/1042_2.png) [@jdelgadozmb](https://forum.microlist.org/u/jdelgadozmb)\ **Post date:** [September 6, 2022, 2:59pm UTC](https://forum.microlist.org/t/96-well-plates-you-would-recommend-for-high-na-imaging/1868/8 "2022-09-06T14:59:24Z")
Details can be found here > **[CLS-AN-437\_DL.pdf](https://www.corning.com/catalog/cls/documents/faqs/CLS-AN-437_DL.pdf)** Skirt Height (mm) 6.096 Here additional dimensions, unfortunately they do not provide a drawing > **[MicroplateDimensions96-384-1536.pdf](https://www.corning.com/catalog/cls/documents/drawings/MicroplateDimensions96-384-1536.pdf)**