# 405-nm excitation conjugated phalloidin

**URL:** <https://forum.microlist.org/t/405-nm-excitation-conjugated-phalloidin/1788>\
**Category:** Probes & Prep\
**Tags:** sim, confocal, actin\
**Created:** [April 25, 2022, 2:10pm UTC](https://forum.microlist.org/t/405-nm-excitation-conjugated-phalloidin/1788 "2022-04-25T14:10:58Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![cleterrier](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/cleterrier/32/38_2.png) [@cleterrier](https://forum.microlist.org/u/cleterrier)\
**Post date:** [April 25, 2022, 2:10pm UTC](https://forum.microlist.org/t/405-nm-excitation-conjugated-phalloidin/1788/1 "2022-04-25T14:10:58Z")

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SIM/confocal folks, what’s the latest on usable fluorescent phalloidin with a 405 nm laser line? Our preferred option (DyLight 405) is not available. AF405+? Atto something? CF405M? iFluor 405? I don’t think Brilliant Violet 421 polymer can be attached to phalloidin.

Figure is from [https://future-science.com/doi/10.2144/btn-2018-0112](https://future-science.com/doi/10.2144/btn-2018-0112) and shows phalloidin conjugated with AF405 (quite bad, we want something better!), AF488, AF55 and AF647.

 ![Capture d’écran 2022-04-25 à 16.09.09](https://us1.discourse-cdn.com/flex020/uploads/microlist/original/1X/c59860a7e8fa1979864f694f6f4003f9b1eebfe3.jpeg)

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**Author:** ![Fluorist](https://sea2.discourse-cdn.com/flex020/user_avatar/forum.microlist.org/fluorist/32/1694_2.png) [@Fluorist](https://forum.microlist.org/u/Fluorist)\
**Post date:** [February 20, 2025, 11:10am UTC](https://forum.microlist.org/t/405-nm-excitation-conjugated-phalloidin/1788/2 "2025-02-20T11:10:50Z")

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Check out Alexa Fluor Plus 405 Phalloidin (6.6nM, 20 minutes in PBS). The 750 version is great too.