# Latest **URL:** https://forum.microlist.org/latest.md?page=2 [Latest](https://forum.microlist.org/latest.md) · [Categories](https://forum.microlist.org/categories.md) · [Tags](https://forum.microlist.org/tags.md) **Page:** 3 --- ## [LSM 7MP scanner calibration fails](https://forum.microlist.org/t/lsm-7mp-scanner-calibration-fails/3249)
**Author:** [@chsm](https://forum.microlist.org/u/chsm)\ **Replies:** 0\ **Last updated:** [October 2, 2025, 7:39pm UTC](https://forum.microlist.org/t/lsm-7mp-scanner-calibration-fails/3249 "2025-10-02T19:39:17Z")
Hi Folks, I reinstalled an old Zeiss LSM 7MP system and aligned the NLO laser. Scanner calibration/scan field test keep failing and I noticed the adjustment laser within the scan head used for these tests may not be ali… --- ## [DeltaVision Elite Microscope (ICCMIC SSD Failure)](https://forum.microlist.org/t/deltavision-elite-microscope-iccmic-ssd-failure/3241)
**Author:** [@Cesar\_Valades](https://forum.microlist.org/u/Cesar_Valades)\ **Replies:** 0\ **Last updated:** [September 29, 2025, 1:48am UTC](https://forum.microlist.org/t/deltavision-elite-microscope-iccmic-ssd-failure/3241 "2025-09-29T01:48:22Z")
Hi all, Recently, we encountered an issue with our DeltaVision Elite microscope: the SSD of the ICCMIC (Windows computer) has failed. Since the system is already more than 10 years old, it is no longer under warranty. … --- ## [To calibrate an eyepiece micrometer!?!](https://forum.microlist.org/t/to-calibrate-an-eyepiece-micrometer/3240)
**Author:** [@peteeliot](https://forum.microlist.org/u/peteeliot)\ **Replies:** 0\ **Last updated:** [September 28, 2025, 1:02pm UTC](https://forum.microlist.org/t/to-calibrate-an-eyepiece-micrometer/3240 "2025-09-28T13:02:34Z")
Hello- I’m new here. Thanks in advance for any help. I am a naturalist. I have a AM dissecting microscope (10-30x) which I use with an eyepiece micrometer to make measurements on dragonfly nymphs. I can calibrate the mi… --- ## [What additional HW do I need for a trinocular Vevor](https://forum.microlist.org/t/what-additional-hw-do-i-need-for-a-trinocular-vevor/3239)
**Author:** [@micke](https://forum.microlist.org/u/micke)\ **Replies:** 9\ **Last updated:** [September 26, 2025, 8:09am UTC](https://forum.microlist.org/t/what-additional-hw-do-i-need-for-a-trinocular-vevor/3239 "2025-09-26T08:09:43Z")
Hello, I am new to the forum and a beginner in microscopy. I bought a VEVOR Composite Triocular Microscope 40X-5000X Biological Microscope and a Lazmin112 Digital Microscope Camera, 8.3 MP 1/1.8 CMOS Sensor 23.2 30-mm. t… --- ## [Artifact when externally triggering Hamamatsu Fusion BT](https://forum.microlist.org/t/artifact-when-externally-triggering-hamamatsu-fusion-bt/3237)
**Author:** [@MattBFly](https://forum.microlist.org/u/MattBFly)\ **Replies:** 4\ **Last updated:** [September 18, 2025, 2:04pm UTC](https://forum.microlist.org/t/artifact-when-externally-triggering-hamamatsu-fusion-bt/3237 "2025-09-18T14:04:58Z")
Hi everyone, I am performing simultaneous patch clamp electrophysiology and widefield, 1p voltage imaging. To synchronize my recordings from these two channels, I am using NI Labview to send a TTL pulse to my Hamamatsu … --- ## [Olympus FV1000 Laser Emission Problem](https://forum.microlist.org/t/olympus-fv1000-laser-emission-problem/3210)
**Author:** [@jfimbery](https://forum.microlist.org/u/jfimbery)\ **Replies:** 6\ **Last updated:** [September 12, 2025, 8:00am UTC](https://forum.microlist.org/t/olympus-fv1000-laser-emission-problem/3210 "2025-09-12T08:00:53Z")
Hi, I am using an Olympus FV1000 confocal microscope. Usually when imaging, there are 2 lights which illuminate and indicate ‘laser emission’ is on. However, I could not get them to work and thus no imaging could occur.… --- ## [Service Provider for Olympus FV1000 Confocal - Scandinavia](https://forum.microlist.org/t/service-provider-for-olympus-fv1000-confocal-scandinavia/3231)
**Author:** [@jfimbery](https://forum.microlist.org/u/jfimbery)\ **Replies:** 0\ **Last updated:** [September 12, 2025, 7:57am UTC](https://forum.microlist.org/t/service-provider-for-olympus-fv1000-confocal-scandinavia/3231 "2025-09-12T07:57:29Z")
Hi, our research group is located in Oslo, Norway. We may need some repair work on an Olympus FV1000 confocal microscope. Anyone have a local suggestion? Naturally, we are hoping to obtain a quote for what the repair wor… --- ## [Dual fluorophore expression in mammalian systems](https://forum.microlist.org/t/dual-fluorophore-expression-in-mammalian-systems/3211)
**Author:** [@Katherineimages](https://forum.microlist.org/u/Katherineimages)\ **Replies:** 6\ **Last updated:** [September 11, 2025, 10:07am UTC](https://forum.microlist.org/t/dual-fluorophore-expression-in-mammalian-systems/3211 "2025-09-11T10:07:30Z")
Hello! I am doing a lot of live mammalian cell imaging with fluorescent proteins but I am struggling to express two fluorescent proteins in my system. One of which is an inducible FP and so I initially tried to optimize… --- ## [Olympus Extended focal imaging (EFI) algorithm](https://forum.microlist.org/t/olympus-extended-focal-imaging-efi-algorithm/3212)
**Author:** [@isv](https://forum.microlist.org/u/isv)\ **Replies:** 3\ **Last updated:** [September 5, 2025, 7:48am UTC](https://forum.microlist.org/t/olympus-extended-focal-imaging-efi-algorithm/3212 "2025-09-05T07:48:22Z")
Dear all, Can anybody explain the algorithm behind the Extended Focal Imaging option available in Olympus CellSens? I understand that it is some form of z-stack projection that is performed before the image is saved, bu… --- ## [Now hiring: Imaging/Microscopy Specialist position at UC Berkeley](https://forum.microlist.org/t/now-hiring-imaging-microscopy-specialist-position-at-uc-berkeley/3208)
**Author:** [@llamero](https://forum.microlist.org/u/llamero)\ **Replies:** 0\ **Last updated:** [August 22, 2025, 8:55pm UTC](https://forum.microlist.org/t/now-hiring-imaging-microscopy-specialist-position-at-uc-berkeley/3208 "2025-08-22T20:55:01Z")
Hey Microscopists, UC Berkeley is now hiring for an imaging specialist at the University of California, Berkeley. The full posting is here and all inquiries and applications are required to go through the portal: I… --- ## [Comparison of different NA from the same magnifications and oil vs no oil for low mags](https://forum.microlist.org/t/comparison-of-different-na-from-the-same-magnifications-and-oil-vs-no-oil-for-low-mags/3198)
**Author:** [@AkiAspiringBiologist](https://forum.microlist.org/u/AkiAspiringBiologist)\ **Replies:** 4\ **Last updated:** [August 20, 2025, 10:59pm UTC](https://forum.microlist.org/t/comparison-of-different-na-from-the-same-magnifications-and-oil-vs-no-oil-for-low-mags/3198 "2025-08-20T22:59:13Z")
Hi there, I’m a hobbyist. More precisely I’m a high-schooler from Austria, about 16 years old, and I have fallen in love with microscopy. I wanted to ask about how different the Numerical Aperture has an effect on the r… --- ## [Frequency Domain FLIM hardware needed](https://forum.microlist.org/t/frequency-domain-flim-hardware-needed/3207)
**Author:** [@And](https://forum.microlist.org/u/And)\ **Replies:** 0\ **Last updated:** [August 17, 2025, 10:56pm UTC](https://forum.microlist.org/t/frequency-domain-flim-hardware-needed/3207 "2025-08-17T22:56:38Z")
Hi all, I have been getting conflicting information on how FD-FLIM works. Particularly relating to the lock-in amplifier. I currently have a system setup for confocal spectroscopy with OBIS lasers and Excelitas SPCMs for… --- ## [Cost-effective, open-source light shutters with Arduino control](https://forum.microlist.org/t/cost-effective-open-source-light-shutters-with-arduino-control/2654)
**Author:** [@fischer](https://forum.microlist.org/u/fischer)\ **Replies:** 3\ **Last updated:** [August 12, 2025, 7:17pm UTC](https://forum.microlist.org/t/cost-effective-open-source-light-shutters-with-arduino-control/2654 "2025-08-12T19:17:35Z")
Microscopes generally employ shutters to limit light exposure for the safety of the samples or the user. Most commercial microscopes come with integrated shutters. For home-built instruments you can purchase external shu… --- ## [How to clean immersion oil from peristaltic pump tubing?](https://forum.microlist.org/t/how-to-clean-immersion-oil-from-peristaltic-pump-tubing/3200)
**Author:** [@Dmltml](https://forum.microlist.org/u/Dmltml)\ **Replies:** 1\ **Last updated:** [August 11, 2025, 4:57pm UTC](https://forum.microlist.org/t/how-to-clean-immersion-oil-from-peristaltic-pump-tubing/3200 "2025-08-11T16:57:57Z")
Hello, I borrowed a peristaltic pump for automatic oil dispension in our system but it has not been used for long time. Some parts are hard to replace so I want to try cleaning it first. Do you have any recommendation w… --- ## [1.35NA vs 1.4NA - for Biological samples](https://forum.microlist.org/t/1-35na-vs-1-4na-for-biological-samples/2798)
**Author:** [@Anatka](https://forum.microlist.org/u/Anatka)\ **Replies:** 6\ **Last updated:** [July 26, 2025, 1:04am UTC](https://forum.microlist.org/t/1-35na-vs-1-4na-for-biological-samples/2798 "2025-07-26T01:04:26Z")
Hello, I am working with a cytigenetic lab, analysing Chromosomes with brightfield microscopes. They just moved from Halogen to LED microsocpes and the team is very uncomfortable with the image. They think that getting … --- ## [FLIM: Easy sample for product demonstrations?](https://forum.microlist.org/t/flim-easy-sample-for-product-demonstrations/3172)
**Author:** [@phoTONIcs](https://forum.microlist.org/u/phoTONIcs)\ **Replies:** 3\ **Last updated:** [July 24, 2025, 8:09am UTC](https://forum.microlist.org/t/flim-easy-sample-for-product-demonstrations/3172 "2025-07-24T08:09:24Z")
Hello Micro-community! Is there an “easy to prep” (non-labor intensive/few reagents) FLIM sample to use to train microscopy users? Thats visually impressive, but gets the job done? --- ## [Recommendation for TiE Transmitted Light LED Illuminator](https://forum.microlist.org/t/recommendation-for-tie-transmitted-light-led-illuminator/3176)
**Author:** [@OConnell](https://forum.microlist.org/u/OConnell)\ **Replies:** 0\ **Last updated:** [July 23, 2025, 2:47pm UTC](https://forum.microlist.org/t/recommendation-for-tie-transmitted-light-led-illuminator/3176 "2025-07-23T14:47:12Z")
Hi All, I’m setting up an old TiE for control with Micro-Manager. I’m looking for a budget-friendly LED replacement for the original transmitted light halogen lamp. I’d be OK using an Arduino to trigger it if that ope… --- ## [How can I run channel-wise acquisition programs on an AxioScan 7?](https://forum.microlist.org/t/how-can-i-run-channel-wise-acquisition-programs-on-an-axioscan-7/3169)
**Author:** [@danlamon](https://forum.microlist.org/u/danlamon)\ **Replies:** 2\ **Last updated:** [July 19, 2025, 8:04pm UTC](https://forum.microlist.org/t/how-can-i-run-channel-wise-acquisition-programs-on-an-axioscan-7/3169 "2025-07-19T20:04:30Z")
Hi everyone, It’s my first post for this forum so please direct me as needed. I’ve run into some photoconversion issues with my slides that are marked for DAPI, AF488 an AF647 so I’m trying to acquire stitched slidesca… --- ## [DIY High Power 4x LED Array on a 30 mm Cage Plate](https://forum.microlist.org/t/diy-high-power-4x-led-array-on-a-30-mm-cage-plate/3167)
**Author:** [@llamero](https://forum.microlist.org/u/llamero)\ **Replies:** 0\ **Last updated:** [July 17, 2025, 1:57am UTC](https://forum.microlist.org/t/diy-high-power-4x-led-array-on-a-30-mm-cage-plate/3167 "2025-07-17T01:57:43Z")
Hey Microscopists, I thought I’d share this project real quick in case some of you find it as useful as we have: GitHub - Llamero/30mm\_cage\_plate\_LED\_MCPCB: LED mount that is compatible with the Thorlabs 30 mm cage plat… --- ## [Olympus FV10MP shutter & AOM problem](https://forum.microlist.org/t/olympus-fv10mp-shutter-aom-problem/3164)
**Author:** [@LCJ](https://forum.microlist.org/u/LCJ)\ **Replies:** 0\ **Last updated:** [July 14, 2025, 1:39pm UTC](https://forum.microlist.org/t/olympus-fv10mp-shutter-aom-problem/3164 "2025-07-14T13:39:54Z")
Hi, I am trying to get an Olympus multiphoton setup from 2009 back into working condition. I would greatly appreciate any help/hints! We can’t control the shutter in front of the laser with the FV10 software, although … --- ## [Coupling lasers to a widefield microscope?](https://forum.microlist.org/t/coupling-lasers-to-a-widefield-microscope/1979)
**Author:** [@Yossi\_Steinberger](https://forum.microlist.org/u/Yossi_Steinberger)\ **Replies:** 8\ **Last updated:** [July 12, 2025, 2:47am UTC](https://forum.microlist.org/t/coupling-lasers-to-a-widefield-microscope/1979 "2025-07-12T02:47:58Z")
Dear μforum - I have a Zeiss Axioobserver microscope, and a EMCCD camera, and wish to purchase a laser light source, and to set up a single molecule imaging system in my lab (I am a PhD student). However, I am ignorant … --- ## [What are the main causes of poor axial resolution in light sheet microscopy? (SPIM)](https://forum.microlist.org/t/what-are-the-main-causes-of-poor-axial-resolution-in-light-sheet-microscopy-spim/3151)
**Author:** [@engpol](https://forum.microlist.org/u/engpol)\ **Replies:** 5\ **Last updated:** [July 10, 2025, 12:29pm UTC](https://forum.microlist.org/t/what-are-the-main-causes-of-poor-axial-resolution-in-light-sheet-microscopy-spim/3151 "2025-07-10T12:29:52Z")
First post on here, apologies beforehand for any mistakes in etiquette. I should also say that I am very new to the field of light sheet microscopy, so please do correct me if anything I say is misguided. P.S Apparently… --- ## [Resealing Scientific Camera](https://forum.microlist.org/t/resealing-scientific-camera/3158)
**Author:** [@Omnistic](https://forum.microlist.org/u/Omnistic)\ **Replies:** 0\ **Last updated:** [July 8, 2025, 2:11pm UTC](https://forum.microlist.org/t/resealing-scientific-camera/3158 "2025-07-08T14:11:48Z")
Hi everyone, Was wondering if anyone ever tried to redo a seal on a scientific camera here. I have a couple of cameras that show signs of condensation, I’m assuming the seal started to leak. I know some manufacturers … --- ## [Parts for discontinued VWR VistaVision inverted scope](https://forum.microlist.org/t/parts-for-discontinued-vwr-vistavision-inverted-scope/3154)
**Author:** [@Sonja](https://forum.microlist.org/u/Sonja)\ **Replies:** 0\ **Last updated:** [June 27, 2025, 9:41pm UTC](https://forum.microlist.org/t/parts-for-discontinued-vwr-vistavision-inverted-scope/3154 "2025-06-27T21:41:10Z")
I have a discontinued VistaVision inverted scope that doesn’t have the mechanical stage or slide holder. Does anyone know of any mechanical stages that can be retrofitted to work with the VWR VistaVision? --- ## [Obtaining parts and information for Nikon Microphot-FXA](https://forum.microlist.org/t/obtaining-parts-and-information-for-nikon-microphot-fxa/2333)
**Author:** [@MicroChemist](https://forum.microlist.org/u/MicroChemist)\ **Replies:** 2\ **Last updated:** [June 17, 2025, 7:59pm UTC](https://forum.microlist.org/t/obtaining-parts-and-information-for-nikon-microphot-fxa/2333 "2025-06-17T19:59:43Z")
I’ve recently obtained a Nikon Microphot-FXA BODY to convert into a microphotographic do it all. One reason I choose this project is my long affiliation with Nikon instruments and Nikon cameras. Although a short, 79pg o… --- ## [Any experience with solvent resistant materials for sample holding in lightsheet](https://forum.microlist.org/t/any-experience-with-solvent-resistant-materials-for-sample-holding-in-lightsheet/89)
**Author:** [@GabyS](https://forum.microlist.org/u/GabyS)\ **Replies:** 7\ **Last updated:** [June 13, 2025, 9:59am UTC](https://forum.microlist.org/t/any-experience-with-solvent-resistant-materials-for-sample-holding-in-lightsheet/89 "2025-06-13T09:59:37Z")
We have the Ultra-microscope II in our facility and would like to 3D print some custom holders. Anyone has experience with good materials to use? We are using for most cases Ethyl cinnamate as imaging media. Thanks --- ## [Hacking and old Leica TCS SP2](https://forum.microlist.org/t/hacking-and-old-leica-tcs-sp2/2077)
**Author:** [@rcphysics](https://forum.microlist.org/u/rcphysics)\ **Replies:** 2\ **Last updated:** [June 9, 2025, 8:48pm UTC](https://forum.microlist.org/t/hacking-and-old-leica-tcs-sp2/2077 "2025-06-09T20:48:39Z")
I received an old Leica setup that our medical school was getting rid of. IT consists of a TCS SP2 scan unit with PMTs and galvo scanners, some lasers, and a DMIRBE. Is there anyone here who has advice for salvaging the… --- ## [LED or lamp light source for BX61 - any suggestions?](https://forum.microlist.org/t/led-or-lamp-light-source-for-bx61-any-suggestions/3134)
**Author:** [@ColinD](https://forum.microlist.org/u/ColinD)\ **Replies:** 0\ **Last updated:** [June 5, 2025, 10:09am UTC](https://forum.microlist.org/t/led-or-lamp-light-source-for-bx61-any-suggestions/3134 "2025-06-05T10:09:58Z")
Dear Microforum We currently use an old metal halide Prior Lumen 200 with an Olympus BX61. When switching off, but not when reducing the power to 0%, there is a 'Bulb error 2" message. The lamp still lights so it’s not … --- ## [Drift in long-term multiposition imaging with Nikon PFS](https://forum.microlist.org/t/drift-in-long-term-multiposition-imaging-with-nikon-pfs/3117)
**Author:** [@cleterrier](https://forum.microlist.org/u/cleterrier)\ **Replies:** 4\ **Last updated:** [May 23, 2025, 11:25am UTC](https://forum.microlist.org/t/drift-in-long-term-multiposition-imaging-with-nikon-pfs/3117 "2025-05-23T11:25:15Z")
Hi, I’m writing here because I know there are good connoisseurs of the Nikon systems around here and hope to get some pointers from you! We are encountering an issue with Z-drift during long-term (\>24 hours) time-lapse… --- ## [Opus 1064 Laser Fiber Coupling Efficiency](https://forum.microlist.org/t/opus-1064-laser-fiber-coupling-efficiency/3119)
**Author:** [@Omnistic](https://forum.microlist.org/u/Omnistic)\ **Replies:** 0\ **Last updated:** [May 22, 2025, 1:20pm UTC](https://forum.microlist.org/t/opus-1064-laser-fiber-coupling-efficiency/3119 "2025-05-22T13:20:32Z")
Hi everyone, DISCLAIMER: do not open a laser case, and if you do make sure you have the right protective equipment, my question is not meant to be a guide on how to fix any particular laser. Do not reproduce the steps I… [Previous page](https://forum.microlist.org/latest.md?page=1) [Next page](https://forum.microlist.org/latest.md?page=3)