# Latest **URL:** https://forum.microlist.org/latest.md [Latest](https://forum.microlist.org/latest.md) · [Categories](https://forum.microlist.org/categories.md) · [Tags](https://forum.microlist.org/tags.md) --- ## [Welcome to Microforum!](https://forum.microlist.org/t/welcome-to-microforum/7)
**Author:** [@system](https://forum.microlist.org/u/system)\ **Replies:** 0\ **Last updated:** [July 9, 2019, 7:11pm UTC](https://forum.microlist.org/t/welcome-to-microforum/7 "2019-07-09T19:11:16Z")
What is Microforum? Microforum’s focus is hardware, acquisition, and specimen-related aspects of scientific imaging, particularly (but not limited to) theory and practical use of optical microscopes and detectors, fluo… --- ## [Equivalent cameras to the Photometrics Kinetix?](https://forum.microlist.org/t/equivalent-cameras-to-the-photometrics-kinetix/3396)
**Author:** [@Kyle\_Douglass](https://forum.microlist.org/u/Kyle_Douglass)\ **Replies:** 8\ **Last updated:** [September 17, 2026, 1:05pm UTC](https://forum.microlist.org/t/equivalent-cameras-to-the-photometrics-kinetix/3396 "2026-09-17T13:05:38Z")
Hi all, I am re-entering the camera market after a long hiatus and am wondering whether any Photometrics competitors offer a camera that is the equivalent of or similar to the Kinetix? I am assembling a spreadsheet of … --- ## [Andor Neo throws an error \[micro-manager\]](https://forum.microlist.org/t/andor-neo-throws-an-error-micro-manager/3410)
**Author:** [@Daniel-Waiger](https://forum.microlist.org/u/Daniel-Waiger)\ **Replies:** 1\ **Last updated:** [September 15, 2026, 12:53pm UTC](https://forum.microlist.org/t/andor-neo-throws-an-error-micro-manager/3410 "2026-09-15T12:53:44Z")
Hi All, My camera decided to spite me, on my last PhD exp. I get this error and not sure what to do. I reset the PC, camera, and microscope (Nikon Eclipse Ti). USBs are plugged-in, and basically nothing changed in the … --- ## [Best open software for optical system design](https://forum.microlist.org/t/best-open-software-for-optical-system-design/353)
**Author:** [@hgibbs](https://forum.microlist.org/u/hgibbs)\ **Replies:** 25\ **Last updated:** [September 13, 2026, 2:02am UTC](https://forum.microlist.org/t/best-open-software-for-optical-system-design/353 "2026-09-13T02:02:27Z")
I’ve only used Zemax before, but unfortunately have no license at the moment. Is there anyone out there with a positive experience learning/using an open source tool? --- ## [Micronaut: a browser-based planner for microscopy experiments](https://forum.microlist.org/t/micronaut-a-browser-based-planner-for-microscopy-experiments/3409)
**Author:** [@Daniel-Waiger](https://forum.microlist.org/u/Daniel-Waiger)\ **Replies:** 0\ **Last updated:** [September 9, 2026, 11:00am UTC](https://forum.microlist.org/t/micronaut-a-browser-based-planner-for-microscopy-experiments/3409 "2026-09-09T11:00:03Z")
Hi all, I have been building a small free tool called Micronaut and would value this community’s eyes on it. It is a planner for the stage before acquisition: the point where you decide what you are comparing, what coun… --- ## [Job opportunity: Imaging Scientist, Columbia University](https://forum.microlist.org/t/job-opportunity-imaging-scientist-columbia-university/3408)
**Author:** [@tswayne](https://forum.microlist.org/u/tswayne)\ **Replies:** 0\ **Last updated:** [September 8, 2026, 8:20pm UTC](https://forum.microlist.org/t/job-opportunity-imaging-scientist-columbia-university/3408 "2026-09-08T20:20:19Z")
Please share this opportunity with any interested colleagues. Feel free to contact me with any questions. Theresa ------------------ Position: Associate Research Scientist Location: Columbia University in the City of… --- ## [Manual rotary microtome](https://forum.microlist.org/t/manual-rotary-microtome/3407)
**Author:** [@ChristopheWinckler](https://forum.microlist.org/u/ChristopheWinckler)\ **Replies:** 1\ **Last updated:** [September 8, 2026, 7:56pm UTC](https://forum.microlist.org/t/manual-rotary-microtome/3407 "2026-09-08T19:56:52Z")
I have a manual rotary microtome and I do not use it anymore. Who can help me on this topic., --- ## [Long-term Live Cell Imaging tips/tricks](https://forum.microlist.org/t/long-term-live-cell-imaging-tips-tricks/70)
**Author:** [@Maikel](https://forum.microlist.org/u/Maikel)\ **Replies:** 23\ **Last updated:** [September 4, 2026, 10:49am UTC](https://forum.microlist.org/t/long-term-live-cell-imaging-tips-tricks/70 "2026-09-04T10:49:32Z")
Hello – My project requires me to image cultured cells (macrophages) for up to 72 hours on an epi-fluorescence microscope. Are there any specific tips and tricks that people have that I should consider when doing these e… --- ## [Digital Offset settings Confocal PMT](https://forum.microlist.org/t/digital-offset-settings-confocal-pmt/1372)
**Author:** [@Mitchell\_Han](https://forum.microlist.org/u/Mitchell_Han)\ **Replies:** 9\ **Last updated:** [August 25, 2026, 11:57am UTC](https://forum.microlist.org/t/digital-offset-settings-confocal-pmt/1372 "2026-08-25T11:57:42Z")
Dear all, I recently watched the excellent training video for the LSM880 by Jason Kirk for reference of our own facility. In his video he mentions using the Digital Offset slider for the PMT to eliminate “blue” (underex… --- ## [Discrepancy in root anatomy measurements between compound and dissecting microscopes at the same magnification (4x)?](https://forum.microlist.org/t/discrepancy-in-root-anatomy-measurements-between-compound-and-dissecting-microscopes-at-the-same-magnification-4x/3399)
**Author:** [@Ishrat\_Zahan](https://forum.microlist.org/u/Ishrat_Zahan)\ **Replies:** 5\ **Last updated:** [August 21, 2026, 8:30pm UTC](https://forum.microlist.org/t/discrepancy-in-root-anatomy-measurements-between-compound-and-dissecting-microscopes-at-the-same-magnification-4x/3399 "2026-08-21T20:30:44Z")
Hello everyone, I am measuring wheat root anatomical traits (whole root diameter, stele diameter, etc.) using ImageJ. Initially, I used a compound microscope at 4x magnification and calibrated the scale using a physical … --- ## [Nikon Eclipse Ti back port to Back Focal Plane distance specification needed](https://forum.microlist.org/t/nikon-eclipse-ti-back-port-to-back-focal-plane-distance-specification-needed/3204)
**Author:** [@Yossi\_Steinberger](https://forum.microlist.org/u/Yossi_Steinberger)\ **Replies:** 10\ **Last updated:** [August 17, 2026, 7:11am UTC](https://forum.microlist.org/t/nikon-eclipse-ti-back-port-to-back-focal-plane-distance-specification-needed/3204 "2026-08-17T07:11:48Z")
Hello all, I am designing a single-molecule microscope using a Nikon Eclipse Ti-E inverted microscope. My setup involves focusing a collimated laser beam onto the back focal plane of a 100×/1.4 NA objective via a ~200mm… --- ## [Fixing broken pins/contacts on a ZEISS Lattice Lightsheet sample chamber lid](https://forum.microlist.org/t/fixing-broken-pins-contacts-on-a-zeiss-lattice-lightsheet-sample-chamber-lid/3397)
**Author:** [@Omnistic](https://forum.microlist.org/u/Omnistic)\ **Replies:** 0\ **Last updated:** [August 7, 2026, 2:57pm UTC](https://forum.microlist.org/t/fixing-broken-pins-contacts-on-a-zeiss-lattice-lightsheet-sample-chamber-lid/3397 "2026-08-07T14:57:15Z")
Hello everyone, In our facility, the sample chamber lid of our ZEISS Lattice Lightsheet had some broken pins/contacts. We do not know how it happened, but some pins were stuck at their minimum height and weren’t contact… --- ## [Problem with Zeiss LSM 800 + Airyscan: No Confocal Communication in ZEN 3.3](https://forum.microlist.org/t/problem-with-zeiss-lsm-800-airyscan-no-confocal-communication-in-zen-3-3/3203)
**Author:** [@saltfishpoi](https://forum.microlist.org/u/saltfishpoi)\ **Replies:** 4\ **Last updated:** [August 6, 2026, 7:32pm UTC](https://forum.microlist.org/t/problem-with-zeiss-lsm-800-airyscan-no-confocal-communication-in-zen-3-3/3203 "2026-08-06T19:32:21Z")
Hello everyone, Our lab was recently reorganized, and we have set up a Zeiss LSM 800 with an Airyscan detector. After some effort, we located the original control computer that was in storage and are now facing a commun… --- ## [Connection error for DeltaVision Elite microscope](https://forum.microlist.org/t/connection-error-for-deltavision-elite-microscope/3017)
**Author:** [@Lanxin\_Bei](https://forum.microlist.org/u/Lanxin_Bei)\ **Replies:** 8\ **Last updated:** [August 5, 2026, 3:32pm UTC](https://forum.microlist.org/t/connection-error-for-deltavision-elite-microscope/3017 "2026-08-05T15:32:05Z")
Hi all, I am a DeltaVision Elite user experiencing a connection issue. I would appreciate any answers or suggestions from our community. Here is a brief description of the error. The blue IC/MIC bottom can not be turn… --- ## [LSM 780 Spectral Detector Servo Motor Map](https://forum.microlist.org/t/lsm-780-spectral-detector-servo-motor-map/3394)
**Author:** [@llamero](https://forum.microlist.org/u/llamero)\ **Replies:** 1\ **Last updated:** [August 3, 2026, 2:35pm UTC](https://forum.microlist.org/t/lsm-780-spectral-detector-servo-motor-map/3394 "2026-08-03T14:35:11Z")
Hey Microscopists, In case this is helpful to anyone else, I went ahead and mapped out the motors in the spectral detector for the LSM 780: LSM 780 Spectral Detector Motor Map.pptx - Google Slides Cheers, Ben Smith --- ## [Auto focus device integration](https://forum.microlist.org/t/auto-focus-device-integration/3395)
**Author:** [@Sanjay\_Tyagi](https://forum.microlist.org/u/Sanjay_Tyagi)\ **Replies:** 0\ **Last updated:** [August 3, 2026, 2:02am UTC](https://forum.microlist.org/t/auto-focus-device-integration/3395 "2026-08-03T02:02:02Z")
I am exploring if it is possible to integrate a Prior PureFocus850 autofocus device into a Zeiss Axiovert 200M microscope. It is controlled by MetaMorph on Windows 7. If anybody has experience with this exact combination… --- ## [Looking for relicts of Leica TCS-SP2](https://forum.microlist.org/t/looking-for-relicts-of-leica-tcs-sp2/2422)
**Author:** [@blaubaer](https://forum.microlist.org/u/blaubaer)\ **Replies:** 5\ **Last updated:** [July 17, 2026, 10:32am UTC](https://forum.microlist.org/t/looking-for-relicts-of-leica-tcs-sp2/2422 "2026-07-17T10:32:27Z")
25 year old Leica TCS SP2 is far out of professional awareness but it may be still charming for hobby use. I have collected the AOTF-scanhead, supply station plus other hardware complete and the right LCS version as well… --- ## [How perfect is Nikon's Perfect Focus System (PFS)?](https://forum.microlist.org/t/how-perfect-is-nikons-perfect-focus-system-pfs/2610)
**Author:** [@georgeoshardo](https://forum.microlist.org/u/georgeoshardo)\ **Replies:** 15\ **Last updated:** [July 14, 2026, 10:35am UTC](https://forum.microlist.org/t/how-perfect-is-nikons-perfect-focus-system-pfs/2610 "2026-07-14T10:35:02Z")
Dear all, I have been dealing with a sporadic problem with our Nikon TI2-Eclipse’s perfect focus system for some time now. My issues always arise when doing very large and long timelapse experiments. Here is my typical … --- ## [Zeiss AxiObserver Main controller2 board -PmcMain.exe, xml exception error](https://forum.microlist.org/t/zeiss-axiobserver-main-controller2-board-pmcmain-exe-xml-exception-error/3391)
**Author:** [@GGasp](https://forum.microlist.org/u/GGasp)\ **Replies:** 0\ **Last updated:** [July 8, 2026, 5:44pm UTC](https://forum.microlist.org/t/zeiss-axiobserver-main-controller2-board-pmcmain-exe-xml-exception-error/3391 "2026-07-08T17:44:22Z")
Hi all, Hoping someone here has hit this specific failure before, or has hands-on experience with the MainController2 board internals. System: Zeiss Axio Observer.Z1 Controller board: Garz & Fricke MainControlle… --- ## [Mitobrilliant stain on FFPE tissue](https://forum.microlist.org/t/mitobrilliant-stain-on-ffpe-tissue/3390)
**Author:** [@DaniilShabashvili](https://forum.microlist.org/u/DaniilShabashvili)\ **Replies:** 0\ **Last updated:** [July 2, 2026, 8:18pm UTC](https://forum.microlist.org/t/mitobrilliant-stain-on-ffpe-tissue/3390 "2026-07-02T20:18:50Z")
Has anyone tried mitobrilliant stain on FFPE tissue? We have a client who wants to do this but can’t find anything in literature. Thank you! --- ## [Staining live/dead with organoids embedded in Matrigel](https://forum.microlist.org/t/staining-live-dead-with-organoids-embedded-in-matrigel/3389)
**Author:** [@DaniilShabashvili](https://forum.microlist.org/u/DaniilShabashvili)\ **Replies:** 1\ **Last updated:** [July 2, 2026, 8:17pm UTC](https://forum.microlist.org/t/staining-live-dead-with-organoids-embedded-in-matrigel/3389 "2026-07-02T20:17:33Z")
We have a user trying to use this live-dead kit to stain cells in a layer of Matrigel, then image with a Keyence microscope LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells 1 kit | Buy Online His staining does… --- ## [Leitz Laborlux D](https://forum.microlist.org/t/leitz-laborlux-d/3387)
**Author:** [@Igo](https://forum.microlist.org/u/Igo)\ **Replies:** 0\ **Last updated:** [June 18, 2026, 2:25pm UTC](https://forum.microlist.org/t/leitz-laborlux-d/3387 "2026-06-18T14:25:42Z")
Hello, I have a Leitz Laborlux D here with a fluorescent HMI ballast, a phototube, and a Ploemopak. Can anyone tell me more about this microscope? I’d like to start doing microscopy again after many years. Can you recom… --- ## [Nikon Microphot-FXA project / Hardware Parts](https://forum.microlist.org/t/nikon-microphot-fxa-project-hardware-parts/3060)
**Author:** [@aristillius](https://forum.microlist.org/u/aristillius)\ **Replies:** 12\ **Last updated:** [June 16, 2026, 3:09am UTC](https://forum.microlist.org/t/nikon-microphot-fxa-project-hardware-parts/3060 "2026-06-16T03:09:06Z")
Continuing the discussions from Obtaining parts and information for Nikon Microphot-FXA and Nikon Microphot-FXA Microscope: Mike, (@Mdevvy) Thanks for the status on your project. I figured I would start new conversat… --- ## [Nikon Microphot-FXA Microscope](https://forum.microlist.org/t/nikon-microphot-fxa-microscope/2588)
**Author:** [@MicroChemist](https://forum.microlist.org/u/MicroChemist)\ **Replies:** 7\ **Last updated:** [June 15, 2026, 4:44am UTC](https://forum.microlist.org/t/nikon-microphot-fxa-microscope/2588 "2026-06-15T04:44:57Z")
Hi! I have a Nikon Microphot-FXA Microscope. There looks to be a large slider hole between the nose piece and the EPI-FL3 Filter box not listed in the operations manual. Does anyone have any information on wh… --- ## [Zeiss Ultrafluor lenses -](https://forum.microlist.org/t/zeiss-ultrafluor-lenses/3381)
**Author:** [@nodi75](https://forum.microlist.org/u/nodi75)\ **Replies:** 4\ **Last updated:** [June 11, 2026, 4:26pm UTC](https://forum.microlist.org/t/zeiss-ultrafluor-lenses/3381 "2026-06-11T16:26:12Z")
Hi all, quick question on this series of lenses - are they still actively used or primarily of historic interest? What is the basic equipment required to assess their functional quality / degradation? Are they hard to fi… --- ## [Anyone have their favorite HCI?](https://forum.microlist.org/t/anyone-have-their-favorite-hci/3301)
**Author:** [@JDP97](https://forum.microlist.org/u/JDP97)\ **Replies:** 3\ **Last updated:** [May 30, 2026, 1:44pm UTC](https://forum.microlist.org/t/anyone-have-their-favorite-hci/3301 "2026-05-30T13:44:31Z")
Hi Everyone! I have the privilege of purchasing a new HCI. I have been looking at a few different options and have a budget of around $500k. Does anyone have their favorite? I have used the CX7 LZR Pro extensively in the… --- ## [DAPI in plant tissue - nonspecific binding to cell walls and chloroplasts](https://forum.microlist.org/t/dapi-in-plant-tissue-nonspecific-binding-to-cell-walls-and-chloroplasts/3346)
**Author:** [@renata\_schnablova](https://forum.microlist.org/u/renata_schnablova)\ **Replies:** 0\ **Last updated:** [May 19, 2026, 6:25pm UTC](https://forum.microlist.org/t/dapi-in-plant-tissue-nonspecific-binding-to-cell-walls-and-chloroplasts/3346 "2026-05-19T18:25:28Z")
Dear all. I am facing difficulties with staining of fixed plant tissue in diverse herbaceous species. In some species (about 10% of the studied species), I see nonspecific staining of cell walls and chloroplasts, withou… --- ## [Camera orientation handling in Micro-Manager and pymmcoreplus](https://forum.microlist.org/t/camera-orientation-handling-in-micro-manager-and-pymmcoreplus/3328)
**Author:** [@CellKai](https://forum.microlist.org/u/CellKai)\ **Replies:** 1\ **Last updated:** [April 29, 2026, 7:46pm UTC](https://forum.microlist.org/t/camera-orientation-handling-in-micro-manager-and-pymmcoreplus/3328 "2026-04-29T19:46:09Z")
I have a working MM config that controls a NikonTi2 CSU-W1 spinning disk confocal system dual cameras. For my application, I am using pymmcore(-plus) to load this configuration and then operate the microscope using custo… --- ## [Materials Science (Metallography) with a Biological microscope](https://forum.microlist.org/t/materials-science-metallography-with-a-biological-microscope/3330)
**Author:** [@Dimomo](https://forum.microlist.org/u/Dimomo)\ **Replies:** 2\ **Last updated:** [April 29, 2026, 7:45pm UTC](https://forum.microlist.org/t/materials-science-metallography-with-a-biological-microscope/3330 "2026-04-29T19:45:49Z")
This device was developed for “epi illumination on a Biological microscope (trans illuminated)”. Since biological (compound) microscopes represent 70 to 80% of all microscopes in existence (source: ChatGPT) an accessory … --- ## [DIY Epifluorescence Macroscope](https://forum.microlist.org/t/diy-epifluorescence-macroscope/1519)
**Author:** [@llamero](https://forum.microlist.org/u/llamero)\ **Replies:** 5\ **Last updated:** [April 26, 2026, 5:25pm UTC](https://forum.microlist.org/t/diy-epifluorescence-macroscope/1519 "2026-04-26T17:25:05Z")
Hey Microscopists, I recently designed and built an epifluorescence macroscope centered around the Olympus XLFLUOR 4x/0.28NA objective and Kinetix camera for both fixed and live imaging. It uses the high power CBT-90 L… [Next page](https://forum.microlist.org/latest.md?page=1)