# Uncategorized **URL:** https://forum.microlist.org/c/uncategorized/1.md?page=2 [Latest](https://forum.microlist.org/latest.md) · [Categories](https://forum.microlist.org/categories.md) · [Tags](https://forum.microlist.org/tags.md) **Page:** 3 --- ## [Mounting media and fluorophore compatibility](https://forum.microlist.org/t/mounting-media-and-fluorophore-compatibility/898)
**Author:** [@LPUoO](https://forum.microlist.org/u/LPUoO)\ **Replies:** 2\ **Last updated:** [August 4, 2020, 3:31pm UTC](https://forum.microlist.org/t/mounting-media-and-fluorophore-compatibility/898 "2020-08-04T15:31:20Z")
Hi, Some report that some mounting media are not compatible with some fluorophores. For example possibly Vectashield is not compatible with far red fluorophores. Is this correct ? or is this specific possibly to only o… --- ## [Capture 3D images of mouse](https://forum.microlist.org/t/capture-3d-images-of-mouse/1054)
**Author:** [@monajalal](https://forum.microlist.org/u/monajalal)\ **Replies:** 5\ **Last updated:** [June 30, 2020, 8:28pm UTC](https://forum.microlist.org/t/capture-3d-images-of-mouse/1054 "2020-06-30T20:28:39Z")
I am interested in a setup that I could capture 3D images of mouse. What setup of two cameras would be ideal for this capture? Could you please point me to a couple of preferred research articles that you see apt for thi… --- ## [Hygienic Practices at the Scopes Template](https://forum.microlist.org/t/hygienic-practices-at-the-scopes-template/914)
**Author:** [@nranthony](https://forum.microlist.org/u/nranthony)\ **Replies:** 6\ **Last updated:** [June 21, 2020, 9:26am UTC](https://forum.microlist.org/t/hygienic-practices-at-the-scopes-template/914 "2020-06-21T09:26:07Z")
Hi all, please see below link for a template for printing and posting near your systems. Fill in the facility name and email, right click and replace logo at the bottom. Please let me know if you have any feedback, an… --- ## [Please help: Collaborative Standards for 3D BRAIN Microscopy](https://forum.microlist.org/t/please-help-collaborative-standards-for-3d-brain-microscopy/954)
**Author:** [@Jan](https://forum.microlist.org/u/Jan)\ **Replies:** 5\ **Last updated:** [June 19, 2020, 3:50pm UTC](https://forum.microlist.org/t/please-help-collaborative-standards-for-3d-brain-microscopy/954 "2020-06-19T15:50:59Z")
Hi everyone, I have been part of a workgroup on collaborative standards for 3D BRAIN microscopy. We have been working hard on finding standards for tissue clearing protocols and for microscopy metadata. Now has come the… --- ## [Laser Curtain Recommendations](https://forum.microlist.org/t/laser-curtain-recommendations/1006)
**Author:** [@nranthony](https://forum.microlist.org/u/nranthony)\ **Replies:** 2\ **Last updated:** [June 3, 2020, 5:29pm UTC](https://forum.microlist.org/t/laser-curtain-recommendations/1006 "2020-06-03T17:29:06Z")
Hi all, hope you are safe and sane. (Repeat of post on BINA Slack https://bioimagingna.slack.com/archives/C9H8SGRT8/p1589999513002700, as an FYI) Does anybody have any recommendations for laser curtain companies, or sp… --- ## [Culture cell medium influence on fluorescence or SHG and THG](https://forum.microlist.org/t/culture-cell-medium-influence-on-fluorescence-or-shg-and-thg/928)
**Author:** [@Alex.h](https://forum.microlist.org/u/Alex.h)\ **Replies:** 1\ **Last updated:** [April 18, 2020, 2:29am UTC](https://forum.microlist.org/t/culture-cell-medium-influence-on-fluorescence-or-shg-and-thg/928 "2020-04-18T02:29:53Z")
Hello everybodies, Do you know if medium or phenol red can have an influence on fluorescence or second harmonic or third harmonic generation?? kind regards --- ## [Cleaning a lens covered in nail polish ;\_;](https://forum.microlist.org/t/cleaning-a-lens-covered-in-nail-polish/813)
**Author:** [@MJS88](https://forum.microlist.org/u/MJS88)\ **Replies:** 9\ **Last updated:** [February 18, 2020, 8:18pm UTC](https://forum.microlist.org/t/cleaning-a-lens-covered-in-nail-polish/813 "2020-02-18T20:18:05Z")
Hi all, A user on my lab’s inverted widefield scope decided to ignore all of my instructions on proper lens care and doused our 100x in nail polish used to seal coverslips (I think–it wasn’t oil and seemed to dry hard).… --- ## [Quantification of double IHC staining (purple and yellow )](https://forum.microlist.org/t/quantification-of-double-ihc-staining-purple-and-yellow/861)
**Author:** [@jeffersonyujl](https://forum.microlist.org/u/jeffersonyujl)\ **Replies:** 1\ **Last updated:** [February 7, 2020, 11:25pm UTC](https://forum.microlist.org/t/quantification-of-double-ihc-staining-purple-and-yellow/861 "2020-02-07T23:25:34Z")
I am running double staining with purple color and yellow color and blue for nucleus staining. My question is to find out if 1,there are more purple and yellows ( in terms of both positive cell number and staining int… --- ## [Cell counting with ImageJ](https://forum.microlist.org/t/cell-counting-with-imagej/817)
**Author:** [@Katie\_Lui](https://forum.microlist.org/u/Katie_Lui)\ **Replies:** 2\ **Last updated:** [January 21, 2020, 2:21pm UTC](https://forum.microlist.org/t/cell-counting-with-imagej/817 "2020-01-21T14:21:39Z")
I was trying to do cell counting analysis on some of my cultured rat astrocytes whose boundaries are quite indistinct as the cells are flat and confluent, by watershed after having done some adjustment to the images. The… --- ## [PhD or Postdoc position in lightsheet microscopy](https://forum.microlist.org/t/phd-or-postdoc-position-in-lightsheet-microscopy/819)
**Author:** [@Koen\_Oost](https://forum.microlist.org/u/Koen_Oost)\ **Replies:** 0\ **Last updated:** [January 21, 2020, 1:34pm UTC](https://forum.microlist.org/t/phd-or-postdoc-position-in-lightsheet-microscopy/819 "2020-01-21T13:34:27Z")
Open position in Liberali group www.fmi.ch/opening --- ## [Area changes with Threshold change in ImageJ?](https://forum.microlist.org/t/area-changes-with-threshold-change-in-imagej/785)
**Author:** [@dschnitzler](https://forum.microlist.org/u/dschnitzler)\ **Replies:** 5\ **Last updated:** [January 14, 2020, 10:18pm UTC](https://forum.microlist.org/t/area-changes-with-threshold-change-in-imagej/785 "2020-01-14T22:18:42Z")
Hi All Just signed up to ask about this: I select my ROI in ImageJ and measure and I get a number for the area (say 114611.775). I then adjust the threshold to cover only the particles I’m interested in measuring, measu… --- ## [Non-Fluorescent Silicon Glue](https://forum.microlist.org/t/non-fluorescent-silicon-glue/750)
**Author:** [@Gideon\_Oluniran](https://forum.microlist.org/u/Gideon_Oluniran)\ **Replies:** 0\ **Last updated:** [December 5, 2019, 12:11pm UTC](https://forum.microlist.org/t/non-fluorescent-silicon-glue/750 "2019-12-05T12:11:28Z")
Good day, Can someone please suggest a non-fluorescent silicon glue? I’ll be using them for imaging on a light sheet microscope (488nm excitation mostlyt) but do not want them interfering with my fluorescent images. Gi… --- ## [Microforum Guidelines](https://forum.microlist.org/t/microforum-guidelines/5)
**Author:** [@jennifer](https://forum.microlist.org/u/jennifer)\ **Replies:** 1\ **Last updated:** [July 9, 2019, 7:11pm UTC](https://forum.microlist.org/t/microforum-guidelines/5 "2019-07-09T19:11:16Z")
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