# Uncategorized **URL:** https://forum.microlist.org/c/uncategorized/1.md [Latest](https://forum.microlist.org/latest.md) · [Categories](https://forum.microlist.org/categories.md) · [Tags](https://forum.microlist.org/tags.md) --- ## [Frequently Asked Questions](https://forum.microlist.org/t/frequently-asked-questions/33)
**Author:** [@jennifer](https://forum.microlist.org/u/jennifer)\ **Replies:** 0\ **Last updated:** [July 12, 2019, 1:42pm UTC](https://forum.microlist.org/t/frequently-asked-questions/33 "2019-07-12T13:42:39Z")
What is the forum’s mission? The goal is to embrace the diversity of the scientific imaging community, while fostering independent learning: Enable people to ask “how do I do X?” without prior knowledge of the topic… --- ## [Welcome to Microforum!](https://forum.microlist.org/t/welcome-to-microforum/7)
**Author:** [@system](https://forum.microlist.org/u/system)\ **Replies:** 0\ **Last updated:** [July 9, 2019, 7:11pm UTC](https://forum.microlist.org/t/welcome-to-microforum/7 "2019-07-09T19:11:16Z")
What is Microforum? Microforum’s focus is hardware, acquisition, and specimen-related aspects of scientific imaging, particularly (but not limited to) theory and practical use of optical microscopes and detectors, fluo… --- ## [Discrepancy in root anatomy measurements between compound and dissecting microscopes at the same magnification (4x)?](https://forum.microlist.org/t/discrepancy-in-root-anatomy-measurements-between-compound-and-dissecting-microscopes-at-the-same-magnification-4x/3399)
**Author:** [@Ishrat\_Zahan](https://forum.microlist.org/u/Ishrat_Zahan)\ **Replies:** 5\ **Last updated:** [August 21, 2026, 8:30pm UTC](https://forum.microlist.org/t/discrepancy-in-root-anatomy-measurements-between-compound-and-dissecting-microscopes-at-the-same-magnification-4x/3399 "2026-08-21T20:30:44Z")
Hello everyone, I am measuring wheat root anatomical traits (whole root diameter, stele diameter, etc.) using ImageJ. Initially, I used a compound microscope at 4x magnification and calibrated the scale using a physical … --- ## [Auto focus device integration](https://forum.microlist.org/t/auto-focus-device-integration/3395)
**Author:** [@Sanjay\_Tyagi](https://forum.microlist.org/u/Sanjay_Tyagi)\ **Replies:** 0\ **Last updated:** [August 3, 2026, 2:02am UTC](https://forum.microlist.org/t/auto-focus-device-integration/3395 "2026-08-03T02:02:02Z")
I am exploring if it is possible to integrate a Prior PureFocus850 autofocus device into a Zeiss Axiovert 200M microscope. It is controlled by MetaMorph on Windows 7. If anybody has experience with this exact combination… --- ## [Byo Soa Lightsource lens blurry](https://forum.microlist.org/t/byo-soa-lightsource-lens-blurry/3309)
**Author:** [@Schakel](https://forum.microlist.org/u/Schakel)\ **Replies:** 1\ **Last updated:** [March 11, 2026, 9:11am UTC](https://forum.microlist.org/t/byo-soa-lightsource-lens-blurry/3309 "2026-03-11T09:11:39Z")
Hello, I am new to this forum but I have a particulair problem I have bought a Byo Soa microscope long time ago but the lens of the light emmiter is getting blurry. This gives a repeating patern in the the oculair len… --- ## [Troubleshooting Two-Photon Excitation With Filipin III Labeling](https://forum.microlist.org/t/troubleshooting-two-photon-excitation-with-filipin-iii-labeling/1421)
**Author:** [@Sveeggen](https://forum.microlist.org/u/Sveeggen)\ **Replies:** 7\ **Last updated:** [March 4, 2026, 2:40am UTC](https://forum.microlist.org/t/troubleshooting-two-photon-excitation-with-filipin-iii-labeling/1421 "2026-03-04T02:40:47Z")
Hello, I’ve been working on imaging cholesterol localization in human endothelial cells using Filipin III. Because the excitation for Filipin is around 350, we have to use Two-photon excitation because no one on campus … --- ## [Readout Noise Non-Uniformities Hamamatsu Fusion BT](https://forum.microlist.org/t/readout-noise-non-uniformities-hamamatsu-fusion-bt/3253)
**Author:** [@Omnistic](https://forum.microlist.org/u/Omnistic)\ **Replies:** 4\ **Last updated:** [January 8, 2026, 10:55am UTC](https://forum.microlist.org/t/readout-noise-non-uniformities-hamamatsu-fusion-bt/3253 "2026-01-08T10:55:53Z")
Hi everyone, I just want to raise awareness about an issue I’ve been having for a long time with Hamamatsu’s ORCA-Fusion BT (C15440-20UP). It is something we already noticed in my previous institute with some colleagues… --- ## [How to use SQUIRREL analysis as a performance evaluation metric?](https://forum.microlist.org/t/how-to-use-squirrel-analysis-as-a-performance-evaluation-metric/3279)
**Author:** [@Qiqi-Lu](https://forum.microlist.org/u/Qiqi-Lu)\ **Replies:** 0\ **Last updated:** [November 26, 2025, 5:15pm UTC](https://forum.microlist.org/t/how-to-use-squirrel-analysis-as-a-performance-evaluation-metric/3279 "2025-11-26T17:15:53Z")
Hi everyone, I want to use RSE and RSP in SQUIRREL analysis as two metrics to evaluate the restoration performance of a deep learning network. Now, I have a WF image (as network input), restored image (output of networ… --- ## [Zeiss Imager.Z1 contrast manager](https://forum.microlist.org/t/zeiss-imager-z1-contrast-manager/3256)
**Author:** [@Corda](https://forum.microlist.org/u/Corda)\ **Replies:** 0\ **Last updated:** [October 16, 2025, 5:27pm UTC](https://forum.microlist.org/t/zeiss-imager-z1-contrast-manager/3256 "2025-10-16T17:27:55Z")
Hello microlist users Would anybody know how to re-fill the the empty contrast manager on the TFT screen? The automatic objective selection works fine as do all other buttons. Thanks and Best regards, Corda --- ## [Microscope camera software](https://forum.microlist.org/t/microscope-camera-software/3250)
**Author:** [@Fried](https://forum.microlist.org/u/Fried)\ **Replies:** 2\ **Last updated:** [October 7, 2025, 11:50am UTC](https://forum.microlist.org/t/microscope-camera-software/3250 "2025-10-07T11:50:13Z")
Can anyone provide me with Swift Easy View software --- ## [Swift Easy View camera](https://forum.microlist.org/t/swift-easy-view-camera/3137)
**Author:** [@judge](https://forum.microlist.org/u/judge)\ **Replies:** 1\ **Last updated:** [October 6, 2025, 9:14pm UTC](https://forum.microlist.org/t/swift-easy-view-camera/3137 "2025-10-06T21:14:51Z")
I have the Swift Easy View camera version 2.1 manual but it doesn’t explain how to set up the “slide show”. Can anyone help? Thanks, Judge --- ## [What additional HW do I need for a trinocular Vevor](https://forum.microlist.org/t/what-additional-hw-do-i-need-for-a-trinocular-vevor/3239)
**Author:** [@micke](https://forum.microlist.org/u/micke)\ **Replies:** 9\ **Last updated:** [September 26, 2025, 8:09am UTC](https://forum.microlist.org/t/what-additional-hw-do-i-need-for-a-trinocular-vevor/3239 "2025-09-26T08:09:43Z")
Hello, I am new to the forum and a beginner in microscopy. I bought a VEVOR Composite Triocular Microscope 40X-5000X Biological Microscope and a Lazmin112 Digital Microscope Camera, 8.3 MP 1/1.8 CMOS Sensor 23.2 30-mm. t… --- ## [1.35NA vs 1.4NA - for Biological samples](https://forum.microlist.org/t/1-35na-vs-1-4na-for-biological-samples/2798)
**Author:** [@Anatka](https://forum.microlist.org/u/Anatka)\ **Replies:** 6\ **Last updated:** [July 26, 2025, 1:04am UTC](https://forum.microlist.org/t/1-35na-vs-1-4na-for-biological-samples/2798 "2025-07-26T01:04:26Z")
Hello, I am working with a cytigenetic lab, analysing Chromosomes with brightfield microscopes. They just moved from Halogen to LED microsocpes and the team is very uncomfortable with the image. They think that getting … --- ## [Cy3/Cy5/Cy7 multi-spectral beads?](https://forum.microlist.org/t/cy3-cy5-cy7-multi-spectral-beads/2597)
**Author:** [@Hazen\_Babcock](https://forum.microlist.org/u/Hazen_Babcock)\ **Replies:** 2\ **Last updated:** [May 10, 2025, 10:53pm UTC](https://forum.microlist.org/t/cy3-cy5-cy7-multi-spectral-beads/2597 "2025-05-10T22:53:49Z")
I am hoping to find a vendor of multi-spectral beads that contain dyes that are similar to Cy3, Cy5 and Cy7. The excitation wavelengths are 560nm, 650nm and 750nm. Unfortunately the tetraspeck beads that I can find don’t… --- ## [Olympus Fv1000MPE-fluoview not communicating with laser system](https://forum.microlist.org/t/olympus-fv1000mpe-fluoview-not-communicating-with-laser-system/3070)
**Author:** [@Saleem\_Yousf](https://forum.microlist.org/u/Saleem_Yousf)\ **Replies:** 0\ **Last updated:** [April 15, 2025, 8:52pm UTC](https://forum.microlist.org/t/olympus-fv1000mpe-fluoview-not-communicating-with-laser-system/3070 "2025-04-15T20:52:10Z")
I’m currently working on an Olympus FV1000MPE multiphoton system. The Mai Tai laser is functional and can be controlled through the Spectra-Physics GUI, but the FluoView software is not communicating with the laser. The … --- ## [Delta Vision Elite Repair in Canada](https://forum.microlist.org/t/delta-vision-elite-repair-in-canada/2831)
**Author:** [@cegydio27](https://forum.microlist.org/u/cegydio27)\ **Replies:** 6\ **Last updated:** [April 4, 2025, 9:28pm UTC](https://forum.microlist.org/t/delta-vision-elite-repair-in-canada/2831 "2025-04-04T21:28:36Z")
Hello, I am aware a forum concerning this topic was started last year. I don’t want to bring up the same issues about lack of service support, etc. I am looking for alternatives sources to diagnose and potentially repai… --- ## [Image Saturation - No blue pixels vs "few" blue pixels](https://forum.microlist.org/t/image-saturation-no-blue-pixels-vs-few-blue-pixels/1296)
**Author:** [@phoTONIcs](https://forum.microlist.org/u/phoTONIcs)\ **Replies:** 12\ **Last updated:** [March 16, 2025, 1:52pm UTC](https://forum.microlist.org/t/image-saturation-no-blue-pixels-vs-few-blue-pixels/1296 "2025-03-16T13:52:23Z")
A majority of core facilities have differing protocols when it comes to setting confocal parameters. According to Leica, you want a few green and a few blue pixels when looking at the LUT. Other protocols/SOPs state you … --- ## [How does PMT creates pixels?](https://forum.microlist.org/t/how-does-pmt-creates-pixels/2838)
**Author:** [@deepak\_jangra](https://forum.microlist.org/u/deepak_jangra)\ **Replies:** 5\ **Last updated:** [February 28, 2025, 4:28pm UTC](https://forum.microlist.org/t/how-does-pmt-creates-pixels/2838 "2025-02-28T16:28:17Z")
How actually the pixels are created by PMT detectors in confocal microscopes? I understand till signal amplification by dynodes inside PMT and voltage generation at Anode, but how this voltage is transformed into Pixels… --- ## [Pinhole alignment!](https://forum.microlist.org/t/pinhole-alignment/2995)
**Author:** [@Deepak\_Jangra1](https://forum.microlist.org/u/Deepak_Jangra1)\ **Replies:** 1\ **Last updated:** [February 14, 2025, 10:10am UTC](https://forum.microlist.org/t/pinhole-alignment/2995 "2025-02-14T10:10:18Z")
I acquired this snap with full pinhole opened, and no samples on stage. Laser used 647nm. Can anyone comment on this? --- ## [Why does binning increase SNR](https://forum.microlist.org/t/why-does-binning-increase-snr/2936)
**Author:** [@Grasswall](https://forum.microlist.org/u/Grasswall)\ **Replies:** 2\ **Last updated:** [December 23, 2024, 3:26am UTC](https://forum.microlist.org/t/why-does-binning-increase-snr/2936 "2024-12-23T03:26:23Z")
There were mentioning that binning increases SNR in CCD cameras, the source is the mirco course video “Signal to Noise Ratio” time stamp 10:15. --- ## [Axioimager Upright Epifluorescent](https://forum.microlist.org/t/axioimager-upright-epifluorescent/2814)
**Author:** [@Dr.Farah](https://forum.microlist.org/u/Dr.Farah)\ **Replies:** 0\ **Last updated:** [September 25, 2024, 8:50am UTC](https://forum.microlist.org/t/axioimager-upright-epifluorescent/2814 "2024-09-25T08:50:14Z")
hello am using nile red dye at low concentration however am not able to see the emission peak ! is there any option I can do to improve this or I must increase the concentration of the dye ? --- ## [Regarding the best all in one microscope/scanner (bright field, fluorescence - including FISH and polarization)](https://forum.microlist.org/t/regarding-the-best-all-in-one-microscope-scanner-bright-field-fluorescence-including-fish-and-polarization/2657)
**Author:** [@louis\_balan](https://forum.microlist.org/u/louis_balan)\ **Replies:** 1\ **Last updated:** [September 5, 2024, 9:37am UTC](https://forum.microlist.org/t/regarding-the-best-all-in-one-microscope-scanner-bright-field-fluorescence-including-fish-and-polarization/2657 "2024-09-05T09:37:05Z")
Hi, everyone I am a newbie to this forum and stumbled upon by chance. I am a pathologist looking for all the following features in a single microscope or scanner (bright filed, fluorescence including FISH and planning … --- ## [FREE Microtutor LIVE Fluorescence Microscopy course!](https://forum.microlist.org/t/free-microtutor-live-fluorescence-microscopy-course/2633)
**Author:** [@jennifer](https://forum.microlist.org/u/jennifer)\ **Replies:** 0\ **Last updated:** [August 22, 2024, 5:48pm UTC](https://forum.microlist.org/t/free-microtutor-live-fluorescence-microscopy-course/2633 "2024-08-22T17:48:24Z")
There is still time to apply for our FREE Microtutor LIVE Fluorescence Microscopy flipped-classroom course, which begins on 9/9/2024! Join us for guided self-learning of theoretical and practical concepts that will help … --- ## [Any old Olympus FV1000 users?](https://forum.microlist.org/t/any-old-olympus-fv1000-users/2176)
**Author:** [@vytas\_z](https://forum.microlist.org/u/vytas_z)\ **Replies:** 7\ **Last updated:** [April 17, 2024, 10:50am UTC](https://forum.microlist.org/t/any-old-olympus-fv1000-users/2176 "2024-04-17T10:50:48Z")
Hoping to get some help getting an old confocal system up and running. Seems like everything powers up, but the issue is the system talking to the PC (it has the Olympus software on the PC, but it does not seem to detect… --- ## [Setting the camera and stage synchronisation in uManager](https://forum.microlist.org/t/setting-the-camera-and-stage-synchronisation-in-umanager/2482)
**Author:** [@Rob\_crick](https://forum.microlist.org/u/Rob_crick)\ **Replies:** 0\ **Last updated:** [April 5, 2024, 11:05am UTC](https://forum.microlist.org/t/setting-the-camera-and-stage-synchronisation-in-umanager/2482 "2024-04-05T11:05:51Z")
Hello all, We’re currently trying to use uManager 2.0 to drive a Nikon TiE, CoolLed PE 300 and a Prime BSI camera for a multi-position time lapse experiment. The set up has been relatively straight forward but we are ru… --- ## [Looking for opinions on brightfield and fluorescence slide scanners](https://forum.microlist.org/t/looking-for-opinions-on-brightfield-and-fluorescence-slide-scanners/1836)
**Author:** [@VetPathologist](https://forum.microlist.org/u/VetPathologist)\ **Replies:** 8\ **Last updated:** [March 12, 2024, 4:53am UTC](https://forum.microlist.org/t/looking-for-opinions-on-brightfield-and-fluorescence-slide-scanners/1836 "2024-03-12T04:53:23Z")
We are a research pathology core facility and we plan to acquire a slide scanner that performs brightfield and fluorescence whole slide imaging on tissues sections. It seems like this topic has not been discussed in dept… --- ## [5 Major Imaging Challenges in Microscopy & how to solve them](https://forum.microlist.org/t/5-major-imaging-challenges-in-microscopy-how-to-solve-them/2243)
**Author:** [@KevinJackson](https://forum.microlist.org/u/KevinJackson)\ **Replies:** 0\ **Last updated:** [August 15, 2023, 4:20am UTC](https://forum.microlist.org/t/5-major-imaging-challenges-in-microscopy-how-to-solve-them/2243 "2023-08-15T04:20:41Z")
Do imaging artifacts affect the performance of your microscopic devices? Then, don’t miss our upcoming live webinar on the “5 major imaging challenges in microscopy and how to avoid them”! Click here to register your s… --- ## [Any experience with imaging U-bottom polystyrene wells?](https://forum.microlist.org/t/any-experience-with-imaging-u-bottom-polystyrene-wells/2205)
**Author:** [@k\_dewa](https://forum.microlist.org/u/k_dewa)\ **Replies:** 5\ **Last updated:** [July 8, 2023, 7:42pm UTC](https://forum.microlist.org/t/any-experience-with-imaging-u-bottom-polystyrene-wells/2205 "2023-07-08T19:42:43Z")
Hello everyone, I’m a biologist and new to this forum. I’ve encountered a microscopy challenge and would appreciate any ideas to tackling it. I need to image bacterial colonies that grow across the curved surface of U-… --- ## [Stitching of tile images Axio imager/Zen blue](https://forum.microlist.org/t/stitching-of-tile-images-axio-imager-zen-blue/2172)
**Author:** [@asmae](https://forum.microlist.org/u/asmae)\ **Replies:** 9\ **Last updated:** [May 18, 2023, 3:29am UTC](https://forum.microlist.org/t/stitching-of-tile-images-axio-imager-zen-blue/2172 "2023-05-18T03:29:09Z")
Hello, I have a problem of images not being stitched very well (please see image). Images are taking by Zeiss Axio imager and stitching is done in Zen blue (3.6). I used to apply the same stitching parameters and nev… --- ## [Sharp/bright fluorescent images vs pale?](https://forum.microlist.org/t/sharp-bright-fluorescent-images-vs-pale/2128)
**Author:** [@asmae](https://forum.microlist.org/u/asmae)\ **Replies:** 3\ **Last updated:** [April 12, 2023, 10:04am UTC](https://forum.microlist.org/t/sharp-bright-fluorescent-images-vs-pale/2128 "2023-04-12T10:04:50Z")
Hello, I have troubles to understand why some fluorescent images look sharp and bright (picture on the left) while others look pale (picture on the right). They were imaged with different microscopes, here are parameter… [Next page](https://forum.microlist.org/c/uncategorized/1.md?page=1)