# Theory **URL:** https://forum.microlist.org/c/theory/7.md?page=1 [Latest](https://forum.microlist.org/latest.md) · [Categories](https://forum.microlist.org/categories.md) · [Tags](https://forum.microlist.org/tags.md) **Page:** 2 --- ## [Doubts on Airyscan](https://forum.microlist.org/t/doubts-on-airyscan/1231)
**Author:** [@lsilva.m](https://forum.microlist.org/u/lsilva.m)\ **Replies:** 1\ **Last updated:** [December 10, 2020, 11:46am UTC](https://forum.microlist.org/t/doubts-on-airyscan/1231 "2020-12-10T11:46:34Z")
\[this is a copy of a post originally posted in image.sc\] Hello, I am a PhD student, and recently begun to use the confocal microscope (LSM710) with Airyscan, and I have some doubts/curiosities—hopefully, you can help m… --- ## [I need to scan quicker . Zeiss Axio Scan Z1](https://forum.microlist.org/t/i-need-to-scan-quicker-zeiss-axio-scan-z1/1136)
**Author:** [@Mehmet\_Ozcan](https://forum.microlist.org/u/Mehmet_Ozcan)\ **Replies:** 3\ **Last updated:** [September 15, 2020, 10:26pm UTC](https://forum.microlist.org/t/i-need-to-scan-quicker-zeiss-axio-scan-z1/1136 "2020-09-15T22:26:46Z")
I use Axio Scan Z1. I scan at 40x, but it is very slow i think. With default options, 2x2 cm tissue takes minimum 15 minutes. And usually 10% of image is blur. I don’t think that is normal. @sebi06 I clean slide before… --- ## [FLIM - Lifetime imaging - effect of multimerization of a fluorescence protein](https://forum.microlist.org/t/flim-lifetime-imaging-effect-of-multimerization-of-a-fluorescence-protein/1122)
**Author:** [@NicoKiaru](https://forum.microlist.org/u/NicoKiaru)\ **Replies:** 3\ **Last updated:** [September 8, 2020, 7:07am UTC](https://forum.microlist.org/t/flim-lifetime-imaging-effect-of-multimerization-of-a-fluorescence-protein/1122 "2020-09-08T07:07:08Z")
Hello everybody, Does anybody know a good resource describing the effect of multimerization (dimerization) on the lifetime of a fluorophore. Something like the theory or the measurement of the lifetime of GFP vs a dimer… --- ## [Phase-contrast images of cell monolayer for estimating integrity of cell-cell junctions](https://forum.microlist.org/t/phase-contrast-images-of-cell-monolayer-for-estimating-integrity-of-cell-cell-junctions/1030)
**Author:** [@Oleg](https://forum.microlist.org/u/Oleg)\ **Replies:** 4\ **Last updated:** [June 15, 2020, 5:21am UTC](https://forum.microlist.org/t/phase-contrast-images-of-cell-monolayer-for-estimating-integrity-of-cell-cell-junctions/1030 "2020-06-15T05:21:59Z")
Hi, I heard somewhere that width of bright areas between cells in phase-contrast images of cells monolayer is somehow connected to the integrity of intercellular junctions (i.e. distance between cell membranes). Unfortu… --- ## [Extinction coefficient & quantum yield units?](https://forum.microlist.org/t/extinction-coefficient-quantum-yield-units/1007)
**Author:** [@LPUoO](https://forum.microlist.org/u/LPUoO)\ **Replies:** 4\ **Last updated:** [May 26, 2020, 9:43pm UTC](https://forum.microlist.org/t/extinction-coefficient-quantum-yield-units/1007 "2020-05-26T21:43:25Z")
Hi, If the extinction coefficient is the amount of light absorbed at a given wavelength, at a given excitation intensity why isn’t it a percentage? Idem for the quantum yield? What are the standard units? Thanks --- ## [Optimal z-step in confocal](https://forum.microlist.org/t/optimal-z-step-in-confocal/838)
**Author:** [@LPUoO](https://forum.microlist.org/u/LPUoO)\ **Replies:** 2\ **Last updated:** [February 7, 2020, 12:44am UTC](https://forum.microlist.org/t/optimal-z-step-in-confocal/838 "2020-02-07T00:44:25Z")
Hi, I’m trying to find out the formula for the optimal z-step in a confocal. The only info I found is that most acquisition software do the calculation for the user. Could someone give indicate to me what it is ? Tha… --- ## [Extracting Super-resolution Structures inside a Single Molecule or Overlapped Molecules from One Blurred Image](https://forum.microlist.org/t/extracting-super-resolution-structures-inside-a-single-molecule-or-overlapped-molecules-from-one-blurred-image/647)
**Author:** [@Edward.Y.Sheffield](https://forum.microlist.org/u/Edward.Y.Sheffield)\ **Replies:** 12\ **Last updated:** [November 3, 2019, 9:36am UTC](https://forum.microlist.org/t/extracting-super-resolution-structures-inside-a-single-molecule-or-overlapped-molecules-from-one-blurred-image/647 "2019-11-03T09:36:11Z")
https://www.biorxiv.org/content/10.1101/798934v1 This study’s main idea (“resolvable condition”) is strongly inspired by and tightly relevant to the imaging conditions of classic super-resolution techniques. It has unde… --- ## [Maximum imaging depth for good STORM or PALM data?](https://forum.microlist.org/t/maximum-imaging-depth-for-good-storm-or-palm-data/90)
**Author:** [@Elizabeth](https://forum.microlist.org/u/Elizabeth)\ **Replies:** 5\ **Last updated:** [August 8, 2019, 10:02am UTC](https://forum.microlist.org/t/maximum-imaging-depth-for-good-storm-or-palm-data/90 "2019-08-08T10:02:37Z")
Realistically, how deep into tissue can you go while still achieving high-quality single molecule localization data? I’m sure this will vary based on the tissue, but just wondering what a reasonable ballpark would be. Th… [Previous page](https://forum.microlist.org/c/theory/7.md)