# Hardware **URL:** https://forum.microlist.org/c/hardware/6.md?page=4 [Latest](https://forum.microlist.org/latest.md) · [Categories](https://forum.microlist.org/categories.md) · [Tags](https://forum.microlist.org/tags.md) **Page:** 5 --- ## [Part search double c-mount adapter for 3DHistech wsi scanner](https://forum.microlist.org/t/part-search-double-c-mount-adapter-for-3dhistech-wsi-scanner/2445)
**Author:** [@dsmlab](https://forum.microlist.org/u/dsmlab)\ **Replies:** 0\ **Last updated:** [March 6, 2024, 9:12pm UTC](https://forum.microlist.org/t/part-search-double-c-mount-adapter-for-3dhistech-wsi-scanner/2445 "2024-03-06T21:12:01Z")
This is a blatant self-interested request. I’m hoping someone here has the “double adapter” part they no longer need, for our “vintage” 3DHistech scanner, and are willing to part with. I ran across the PUMA project (htt… --- ## [How to know if a objetive can be used in PUMA?](https://forum.microlist.org/t/how-to-know-if-a-objetive-can-be-used-in-puma/2437)
**Author:** [@latot](https://forum.microlist.org/u/latot)\ **Replies:** 1\ **Last updated:** [March 1, 2024, 9:13am UTC](https://forum.microlist.org/t/how-to-know-if-a-objetive-can-be-used-in-puma/2437 "2024-03-01T09:13:51Z")
Hi, I has been thinking, PUMA microscope could be very useful for what I want to do, when we reads the docs there is how need to be the objetive: RMS thread Conjugate focal distance of 195 mm Parfocal length of 45 mm … --- ## [Zeiss Laser Current Threshold Reached Error](https://forum.microlist.org/t/zeiss-laser-current-threshold-reached-error/2426)
**Author:** [@kiraheikes](https://forum.microlist.org/u/kiraheikes)\ **Replies:** 1\ **Last updated:** [February 23, 2024, 2:59pm UTC](https://forum.microlist.org/t/zeiss-laser-current-threshold-reached-error/2426 "2024-02-23T14:59:53Z")
Hi all, Has anyone seen this error and do you know what it means? Is the laser dying? “Laser 561nm:ErrLaserCurrentThresholdReached” We cannot figure it out from Googling or searching Zeiss documents. Using a Zeiss LSM… --- ## [Advantages and Disadvantages about Infinity tech](https://forum.microlist.org/t/advantages-and-disadvantages-about-infinity-tech/2423)
**Author:** [@latot](https://forum.microlist.org/u/latot)\ **Replies:** 2\ **Last updated:** [February 22, 2024, 9:22pm UTC](https://forum.microlist.org/t/advantages-and-disadvantages-about-infinity-tech/2423 "2024-02-22T21:22:31Z")
Hi, I was thinking which microscope to buy, I researched a lot, learned the basics about microscopy and when finally reaching a point to know what to buy I found some hard points in how to choose one, and is about he Inf… --- ## [Selecting a new microscope/imaging system](https://forum.microlist.org/t/selecting-a-new-microscope-imaging-system/2409)
**Author:** [@MartinH](https://forum.microlist.org/u/MartinH)\ **Replies:** 4\ **Last updated:** [February 18, 2024, 9:43am UTC](https://forum.microlist.org/t/selecting-a-new-microscope-imaging-system/2409 "2024-02-18T09:43:33Z")
Dear community, I was approached by a colleague for help with selecting a microscope/imaging system for a new lab building. It is meant to be situated in the lab for everyday imaging when you don´t want to/need to go th… --- ## [Black horizontal lines in LSM 880 airy scan](https://forum.microlist.org/t/black-horizontal-lines-in-lsm-880-airy-scan/2305)
**Author:** [@amgenetics](https://forum.microlist.org/u/amgenetics)\ **Replies:** 4\ **Last updated:** [February 8, 2024, 7:45pm UTC](https://forum.microlist.org/t/black-horizontal-lines-in-lsm-880-airy-scan/2305 "2024-02-08T19:45:08Z")
Hi fellow scientist, As you can see, I have a problem with black horizontal lines in my images that appear after I acquire it (they cannot be seen when looking through the microscope). I have tried unidirectional acq… --- ## [Does 2x scan speed + 2x averaging reduce photobleaching compared to 1x scan speed + 1x averaging (no averaging)?](https://forum.microlist.org/t/does-2x-scan-speed-2x-averaging-reduce-photobleaching-compared-to-1x-scan-speed-1x-averaging-no-averaging/2407)
**Author:** [@JelleP](https://forum.microlist.org/u/JelleP)\ **Replies:** 4\ **Last updated:** [February 7, 2024, 12:56pm UTC](https://forum.microlist.org/t/does-2x-scan-speed-2x-averaging-reduce-photobleaching-compared-to-1x-scan-speed-1x-averaging-no-averaging/2407 "2024-02-07T12:56:34Z")
Hi all, New to this forum and excited to have discovered it! I’m wondering if the statement in this post title is correct: If I set up a typical CLSM line scan with 2x averaging, but 2x the scan speed, does that reduc… --- ## [Microscope image doesnt display entire field of view when I switch to fluorescent bulb?](https://forum.microlist.org/t/microscope-image-doesnt-display-entire-field-of-view-when-i-switch-to-fluorescent-bulb/2403)
**Author:** [@Boone\_Jones](https://forum.microlist.org/u/Boone_Jones)\ **Replies:** 1\ **Last updated:** [February 5, 2024, 12:56pm UTC](https://forum.microlist.org/t/microscope-image-doesnt-display-entire-field-of-view-when-i-switch-to-fluorescent-bulb/2403 "2024-02-05T12:56:21Z")
Im using a fluorescent microscope for the first time. When looking at the slide with bright field, I can see the entire image. However, when switching to just viewing using the fluorescent bulb, the image only appears as… --- ## [Calibrating a BX61 issues](https://forum.microlist.org/t/calibrating-a-bx61-issues/2400)
**Author:** [@ColinD](https://forum.microlist.org/u/ColinD)\ **Replies:** 2\ **Last updated:** [February 4, 2024, 10:53pm UTC](https://forum.microlist.org/t/calibrating-a-bx61-issues/2400 "2024-02-04T22:53:48Z")
I’m recommissioning an Olympus BX61 and trying to calibrate the system in the CellSens software. Following the online instructions I first set the z stop (I set it when I felt a piece of paper between the 100x objective … --- ## [Issue with Olympus UPLSAPO60XW Objective: Bubbles Inside Front Lens After One Week](https://forum.microlist.org/t/issue-with-olympus-uplsapo60xw-objective-bubbles-inside-front-lens-after-one-week/2391)
**Author:** [@kvinicki](https://forum.microlist.org/u/kvinicki)\ **Replies:** 0\ **Last updated:** [January 30, 2024, 5:45pm UTC](https://forum.microlist.org/t/issue-with-olympus-uplsapo60xw-objective-bubbles-inside-front-lens-after-one-week/2391 "2024-01-30T17:45:45Z")
Hi, I’m seeking advice regarding a problem with my Olympus UPLSAPO60XW microscope objective (60X water immersion, NA1.2). After only a week of use, I’ve noticed what seems to be bubbles forming inside the front lens. I’… --- ## [1.0x vs 2.0x objective question to image C elegans in a 96-well plate](https://forum.microlist.org/t/1-0x-vs-2-0x-objective-question-to-image-c-elegans-in-a-96-well-plate/2383)
**Author:** [@Ray](https://forum.microlist.org/u/Ray)\ **Replies:** 0\ **Last updated:** [January 27, 2024, 5:55pm UTC](https://forum.microlist.org/t/1-0x-vs-2-0x-objective-question-to-image-c-elegans-in-a-96-well-plate/2383 "2024-01-27T17:55:43Z")
Hi all, I’ve been imaging C. elegans in a 96-well plate with a 1.0x objective using a Leica IC80 HD camera and 1.0x objective, and achieving usable results at the 6x zoom as I only need to measure length and witdth. We… --- ## [Building a vertical magnetic tweezers design difficulty](https://forum.microlist.org/t/building-a-vertical-magnetic-tweezers-design-difficulty/2207)
**Author:** [@riley\_Mcdonald](https://forum.microlist.org/u/riley_Mcdonald)\ **Replies:** 1\ **Last updated:** [January 11, 2024, 4:39pm UTC](https://forum.microlist.org/t/building-a-vertical-magnetic-tweezers-design-difficulty/2207 "2024-01-11T16:39:37Z")
Hello! My name is Riley and I am a physics REU intern at the Catholic University of America. I work in a biophysics lab currently running experiments on DNA strands using magnetic tweezers. We are currently using a horiz… --- ## [Leitz Wetzlar light bulb type](https://forum.microlist.org/t/leitz-wetzlar-light-bulb-type/2348)
**Author:** [@sromes1](https://forum.microlist.org/u/sromes1)\ **Replies:** 0\ **Last updated:** [December 9, 2023, 8:29pm UTC](https://forum.microlist.org/t/leitz-wetzlar-light-bulb-type/2348 "2023-12-09T20:29:14Z")
hi all I have a \[Leitz Wetzlar microscope from the 70’s and its missing the lightbulb anyone know what type it takes …thanks --- ## [Choice of fluorescent Lamp](https://forum.microlist.org/t/choice-of-fluorescent-lamp/2327)
**Author:** [@Julien\_Dumont](https://forum.microlist.org/u/Julien_Dumont)\ **Replies:** 0\ **Last updated:** [November 23, 2023, 9:39am UTC](https://forum.microlist.org/t/choice-of-fluorescent-lamp/2327 "2023-11-23T09:39:55Z")
Hi everyone, Would anyone willing to share some feedback or experience with their fluorescent lamp ? We are looking to buy a new one for a basic widefield (no live application) and are open to any suggestion in this cho… --- ## [Low power Köhler illumination](https://forum.microlist.org/t/low-power-kohler-illumination/2312)
**Author:** [@P\_Tadrous](https://forum.microlist.org/u/P_Tadrous)\ **Replies:** 0\ **Last updated:** [November 12, 2023, 5:31pm UTC](https://forum.microlist.org/t/low-power-kohler-illumination/2312 "2023-11-12T17:31:07Z")
The PUMA with its improved lower collector Köhler system can deliver full Köhler illumination with objectives as low as x2 - as shown here: I am unaware of any other microscope that can provide full Köhler illuminati… --- ## [TIRF laser power (Nikon Ti2 + Manual TIRF module)](https://forum.microlist.org/t/tirf-laser-power-nikon-ti2-manual-tirf-module/2289)
**Author:** [@smson](https://forum.microlist.org/u/smson)\ **Replies:** 1\ **Last updated:** [October 16, 2023, 6:42pm UTC](https://forum.microlist.org/t/tirf-laser-power-nikon-ti2-manual-tirf-module/2289 "2023-10-16T18:42:14Z")
Hello, I’m setting up Nikon Ti2 with a manual TIRF module. I’m using LU-N4 laser launch equipped with four laser lines. The powers at the fiber output are 15-20mW for all four lasers (at 100% setting), but they are only… --- ## [RappOpto Dichroic Stripes](https://forum.microlist.org/t/rappopto-dichroic-stripes/2284)
**Author:** [@SaraR](https://forum.microlist.org/u/SaraR)\ **Replies:** 0\ **Last updated:** [September 29, 2023, 12:40pm UTC](https://forum.microlist.org/t/rappopto-dichroic-stripes/2284 "2023-09-29T12:40:24Z")
Hi all, We have a RappOpto Photomanipulation Unit (UGA-42 Firefly) installed on our Olympus IX83 2-deck Yokogawa W1 Spinning disk. We purchased the Firefly with two dedicated laser lines for photomanipulation: 405nm and… --- ## [dSTORM camera and objective](https://forum.microlist.org/t/dstorm-camera-and-objective/2281)
**Author:** [@szh141](https://forum.microlist.org/u/szh141)\ **Replies:** 4\ **Last updated:** [September 22, 2023, 9:34pm UTC](https://forum.microlist.org/t/dstorm-camera-and-objective/2281 "2023-09-22T21:34:30Z")
Hello all. I have a Leica Thunder Imager with TIRF, 100x N.A. 1.47 and 4 laser lines with power between 100-250mW. I’m thinking to try dSTORM using the system. The camera on the system is Leica K8 sCMOS, 6.5um pixel, 95… --- ## [New device CellEraser to heat adherent cells locally](https://forum.microlist.org/t/new-device-celleraser-to-heat-adherent-cells-locally/2275)
**Author:** [@semliki16](https://forum.microlist.org/u/semliki16)\ **Replies:** 0\ **Last updated:** [September 11, 2023, 9:33pm UTC](https://forum.microlist.org/t/new-device-celleraser-to-heat-adherent-cells-locally/2275 "2023-09-11T21:33:10Z")
I want to introduce our new microscope attachment CellEraser. The physical principle of this device is based on millimeter wave radiation (94 GHz, 3 mm wavelength). It can heat cells locally (spot is 500-1000 micron in d… --- ## [Live cell heat shock on microscope](https://forum.microlist.org/t/live-cell-heat-shock-on-microscope/720)
**Author:** [@Chaowei](https://forum.microlist.org/u/Chaowei)\ **Replies:** 12\ **Last updated:** [September 11, 2023, 9:23pm UTC](https://forum.microlist.org/t/live-cell-heat-shock-on-microscope/720 "2023-09-11T21:23:40Z")
Hi, I’m looking for suggestions on how to induce heat shock in live cells/yeast on a microscope. What device do you use to meet the fast temperature change in order to induce heat shock? I found the VAHEAT system from B… --- ## [Vintage beck microscope any information would be great!](https://forum.microlist.org/t/vintage-beck-microscope-any-information-would-be-great/2273)
**Author:** [@topshop123](https://forum.microlist.org/u/topshop123)\ **Replies:** 0\ **Last updated:** [September 10, 2023, 2:01pm UTC](https://forum.microlist.org/t/vintage-beck-microscope-any-information-would-be-great/2273 "2023-09-10T14:01:18Z")
hi all I cant find any information online about this microscope , so i thought i would ask on the forum , any info would be great all i have is that its made by Beck and has a number 45113 thanks --- ## [dSTORM laser power](https://forum.microlist.org/t/dstorm-laser-power/2250)
**Author:** [@szh141](https://forum.microlist.org/u/szh141)\ **Replies:** 2\ **Last updated:** [September 3, 2023, 8:09pm UTC](https://forum.microlist.org/t/dstorm-laser-power/2250 "2023-09-03T20:09:02Z")
Hello all. I’m trying to revive a dSTORM system using 100mW 640 laser. Can I have some general suggestion on the laser power requirement at the sample plane for ground state depletion? Is 1-2 kW/cm2 measured at the sampl… --- ## [Wide field fluorescence light source focusing on the back focal plane](https://forum.microlist.org/t/wide-field-fluorescence-light-source-focusing-on-the-back-focal-plane/2254)
**Author:** [@szh141](https://forum.microlist.org/u/szh141)\ **Replies:** 3\ **Last updated:** [August 25, 2023, 1:52pm UTC](https://forum.microlist.org/t/wide-field-fluorescence-light-source-focusing-on-the-back-focal-plane/2254 "2023-08-25T13:52:09Z")
Hello all. I’m curious about how to couple the illumination source into the microscope or back focal plane of the objective to create even illumination on the sample plane. First, is it correct that I need to focus a la… --- ## [Procedure to measure illumination field](https://forum.microlist.org/t/procedure-to-measure-illumination-field/2242)
**Author:** [@Yossi\_Steinberger](https://forum.microlist.org/u/Yossi_Steinberger)\ **Replies:** 2\ **Last updated:** [August 22, 2023, 3:24pm UTC](https://forum.microlist.org/t/procedure-to-measure-illumination-field/2242 "2023-08-22T15:24:59Z")
Hello - What is a good sample for measuring the illumination field? The laser on my commercial system - Dragonfly 505 series - is concentrated via a telescoping lens - and should only be illuminating the center 512x512… --- ## [Spinning disk advice](https://forum.microlist.org/t/spinning-disk-advice/2117)
**Author:** [@Elad\_Katz](https://forum.microlist.org/u/Elad_Katz)\ **Replies:** 6\ **Last updated:** [August 16, 2023, 9:04pm UTC](https://forum.microlist.org/t/spinning-disk-advice/2117 "2023-08-16T21:04:24Z")
I am looking to purchase a new spinning disk system to replace our aging CV7000. Does anyone have a favorite system to work with 384-well formats? Thanks --- ## [Available Fast FLIM products](https://forum.microlist.org/t/available-fast-flim-products/2054)
**Author:** [@szh141](https://forum.microlist.org/u/szh141)\ **Replies:** 1\ **Last updated:** [August 16, 2023, 4:24pm UTC](https://forum.microlist.org/t/available-fast-flim-products/2054 "2023-08-16T16:24:30Z")
Greetings all! Can I have your opinions about the fast FLIM products on the market? Leica’s FALCON is really easy to use. Does anyone have experience comparing the available FLIM detectors and vendors? How’s the Power Hy… --- ## [Is Tucsen TrueChrome 4K Pro Digital Camera any good?](https://forum.microlist.org/t/is-tucsen-truechrome-4k-pro-digital-camera-any-good/2234)
**Author:** [@Dionysus\_Infinitum](https://forum.microlist.org/u/Dionysus_Infinitum)\ **Replies:** 0\ **Last updated:** [August 4, 2023, 2:55pm UTC](https://forum.microlist.org/t/is-tucsen-truechrome-4k-pro-digital-camera-any-good/2234 "2023-08-04T14:55:52Z")
Is Tucsen a reputable microscope camera provider? This specific TrueChrome Camera can apparently do livestitching with the built-in Mosaic software, and only needs a monitor and a mouse to run without the need for a sepa… --- ## [Pinhole alignment on Leica TCS-SP5](https://forum.microlist.org/t/pinhole-alignment-on-leica-tcs-sp5/2223)
**Author:** [@ewanfowler](https://forum.microlist.org/u/ewanfowler)\ **Replies:** 0\ **Last updated:** [July 26, 2023, 11:06am UTC](https://forum.microlist.org/t/pinhole-alignment-on-leica-tcs-sp5/2223 "2023-07-26T11:06:15Z")
Hi, Does anyone know if it is possible to align the pinhole on a Leica SP5 confocal microscope? I find a substantial drop (\>50%) in signal going from 2AU to 1AU. I think Leica told me pinhole alignment was only possible… --- ## [Cameras like Orca Lightning?](https://forum.microlist.org/t/cameras-like-orca-lightning/2215)
**Author:** [@vytas\_z](https://forum.microlist.org/u/vytas_z)\ **Replies:** 1\ **Last updated:** [July 20, 2023, 1:54pm UTC](https://forum.microlist.org/t/cameras-like-orca-lightning/2215 "2023-07-20T13:54:33Z")
Hi all, Looking for a low-nois, high QE (at 500-600nm), camera, ideally with ability to achieve 1kHz at 0.5-1MP. Orca Lightning seems to tick some of the boxes, but just wondering if there is something else out there? … --- ## [What is the refractive index or type of glass used for Leica X-tra microscope slides?](https://forum.microlist.org/t/what-is-the-refractive-index-or-type-of-glass-used-for-leica-x-tra-microscope-slides/2208)
**Author:** [@P\_Tadrous](https://forum.microlist.org/u/P_Tadrous)\ **Replies:** 2\ **Last updated:** [July 15, 2023, 9:03am UTC](https://forum.microlist.org/t/what-is-the-refractive-index-or-type-of-glass-used-for-leica-x-tra-microscope-slides/2208 "2023-07-15T09:03:26Z")
Does anyone know what is the refractive index of the glass used for Leica Surgipath X-tra ™ adhesive slides? Alternatively do you know the type of glass used for these (so I can look up the refractive index)? Thanks. [Previous page](https://forum.microlist.org/c/hardware/6.md?page=3) [Next page](https://forum.microlist.org/c/hardware/6.md?page=5)